Micro final
Terms
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- How do you describe a colony? (characteristics)
- Size, color, elevation, shape, surface appearance, consistancy, odor.
- What is confluent growth?
- Colonies of growth that all run together.
- What is a pure culture?
- Media that has only one type of bacteria growing on/in it.
- What are isolated colonies?
- Bacterial colonies away from, or set apart from other growth or colonies on plated media.
- What do you record on Day 1?
- The source of the sample, gram stain results, what it was streaked out on (BAP, CNA, MAC).
- What is recorded on Day 2?
- Colonoy morphology on all plates, presumptive ID, set up confirmatory and sensitivity plates.
- What is recorded on Day 3?
- Positive ID, sensitivity test results (I,R,S).
- What do you use to run a CAMP test?
- Staph aureus
- What is enriched media?
- Media formulated to meet the requirements of fastidious pathogens. They have extra nutrients added (blood, serum, egg).
- Give an example of enriched media.
- BAP, Chocolate
- What is selective media?
- Media that contains antibacterial substances that inhibit or kill all but a few types of bacteria. Facilitate isolation of a particular type from a mixed specimen.
- Give an example of selective media.
- MAC, CNA
- What is differential media?
- Media that allows bacteria to be differentiated into groups by biochemical reactions on the medium.
- Give an example of differential media.
- MAC, Mannitol Salt, BAP
- What is enrichment broth?
- Liquid media that either contain nutrients that encourage the growth of desired bacteria or contain inhibitory substances that supporess competition.
- Give an example of enrichment broth.
- Selenit Broth, Trypitic Soy Broth (TSB)
- Which plate do you read the hemolysis from?
- BAP
- What Gram - swarms?
- Proteus
- What Gram - turns clear agars green?
- Pseudomonas
- What Gram - has no growth on MAC?
- Pasteurella
- Is Staph or Strep catalase positive?
- Staph
- Is staph aureus or staph epi coagulase positive?
- Staph aureus
- Is staph aureus or staph epi MSA positive?
- Staph aureus
- How do you know if the CAMP test is positive or negative?
- Positive has an arrow pointing to the Staph aureus.