Comparison of Immunoassays
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- What are the 5 advantages of latex agglutination?
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1. versatile
2. Rapid
3. no equipment needed
4. Good screening test
5. Long-lived reagents - What are the 2 drawbacks of latex agglutination?
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1. Variability btwn manufacturs
2. Not quantitative. - What are the 3 advantages of hemagg. inhibition?
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1. Reference methodology
2. Good for IgM assays
3. Easy to read - what are the 2 drawbacks to hemagglutination inhibition?
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1. Time consuming
2. Varied sensitivity - what are the 2 advantages of EMIT?
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1. Simple to perform
2. No separation step needed - what are 2 drawbacks of emit?
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1. Limited to low MW antigens
2. Not as sensitive as other EIAs - what are the qualities of ELISA?
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sensitive/specific
-versatile
-long-living reagents
-variety of detection systems
-good for automation
-adaptable for screening
-qualitative OR quantitative - what are the drawbacks of ELISA?
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only cost-effective for batching
-variability of SP carriers
-some agents are carcinogenic or expensive - what are the advantages to direct or indirect IFA?
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=sensitive
-relatively inexpensive
-long-lived reagents
-variety of methods
-sameday results for DFA - what are the drawbacks to IFA?
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-background/nonspcfc fluorscn
-time consuming
-interpretation needs expert - what is the advantage of RIA?
- sensitive
- WHAT ARE THE DRAWBACKS TO Ria?
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-Disposal of reagents
-Expensive - what are the advantages of SPRIA and IRMA?
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-More stable/sensitive/rapid than plain RIA
-measures total amt of antibody
-uses less reagent than RIA - what are the drawbacks of SPRIA and IRMA?
- same as RIA - disposal/cost
- what are the advantages of complement fixation?
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1. Reference method
2. Relatively sensitive
3. Adaptable to many tests - what are the drawbacks to complement fixation?
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-time consuming
-requires many controls
-expensive reagents