chapters 1-8 continued
Terms
undefined, object
copy deck
- give some basic information about process staining
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-the molecules of this dye or stain bind to different structures
-most stains are basic,that is +lycharged which enables them to bind to the bacteria which are negatively charged - what are the 2 types of stains?
- simple stain and differential stain
- what is simple staining intale?
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-uses only one dye
-it shows the size,the shape and the cellular arrangement - what is differential stains?
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-in which two or more dyes are used
-distunguishes between 2 kinds of organisms - what is gram stain?
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its part of the differential staining. three steps to gram staining...
-it uses crystal violet(1st stain, then iodine (2nd stain),mordant and stain it again w/counterstain (3rd stain-safranin) - what does mordant mean in the gram staining?
- a chemical used to retain stain in certain cells, a decolorizer
- what is a gram (G+) organism
- these organisms retain the violet stain in presence fo a decolorizer.
- what is a gram (G-) organism
- these organisms when decolorized, they lose their crystal violet stain color,now they don't have any color so when they are stained with the safranin dye counterstain)they stain red.
- what happens when a stain is called gram nonreactive?
- no stain or poorly
- what happens to a gram varible
- stain unevenly
- what happens in acid stain
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-dye is retained by some organisms and not by others
-the ones that it is called acid fast - what are the special stains (differential stains)
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-flagellar stain
-spore stain
-negative stain - flagellar stain
- do not stain very well- difficult stain-stain flagella
- spore stain
- in rod shape bacteria-force bacteria to form spores- stain endospores
- negative stain
- these stain the background