Biology final
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- Stole pictures from Franklin and won the nobel prize
- Watson and Crick
- Water molecules tend to stick together
- cohesion
- 2 daughter cells have been formed
- telophase
- DNA containing genes from different organisms/species
- Recombinant DNA
- Most structurally complex molecule known (most complicated of the macro-molecules)
- Proteins
- Carries amino acids to ribosomes
- Transfer RNA (tRNA)
- Function: store genetic materical, transfer genetic material
- Nucleic acids
- Different flowers provide pollen and eggs (done by hand)
- Cross-fertilization
- Intracellular messengers, energy transfer molecules, assist enzyme action: functions of __________
- nucleotides
- At the end of meiosis, there are __ cells
- 4
- Actual combination of alleles carried by an individual (PP, Pp, pp)
- Genotype
- Steps of interphase
- G1 Phase, S Phase, G2 Phase
- If damage on skin from UV Rays is great enough, cell will
- commit suicide
- Necessary for reproduction
- Steroids
- Germ cells undergo ____
- meiosis
- Chromosomes contain:
- Linear DNA strand, Histones
- Tendency for water to stick to walls of surfaces
- adhesion
- All material and structures that lie inside of the plasma membrane, except for the nucleus (cytosol, salts and organic materials, organelles)
- Cytoplasm
- Normal dominant/recessive relationships exist for alleles on X chromosomes in _____
- females
- Fusion of 2 gametes to form a zygote
- Fertilization
- In animals, meiosis always results in the formation of _______
- gametes
- A fat replacement that cannot be digested by humans
- olestra
- Ribosomes attached to this are the ones who are doing their job: making protein
- Endoplasmic Reticulum
- All proteins have this element as a component
- nitrogen
- Molecule that carries the blueprint of life
- DNA
- Accumulation of mutations change organisms to the point where they diverge
- molecular view
- When bad guys leave traces at crime scenes, this is what forensic scientists amplify the traces with to make them usable
- PCR (polymerase chain reaction makes copies of dna)
- Functional segment of DNA located at a particular place on a chromosome
- Gene
- Homologous chromosomes pair up and separate from one another (2n --> 1n)
- Meiosis I
- All macromolecules have this element as a component (9.5% of human body)
- hydrogen
- DNA Backbone
- Deoxyribose, phosphate
- Packaging proteins (helps chromosomes condense)
- Histones
- Same flower provides pollen and eggs
- self-fertilization
- Number of protons and neutrons in nucleus
- Atomic mass
- Very first dna case
- 1988: colin pitchfork (pedophile that killed 2 young girls in england)
- This part of the ER is rough because these are attached to it
- ribosomes
- Synthesizes cellulose and pectin (plants)
- Golgi apparatus
- A membrane bound nucleus, DNA that is contained within the nucleus, Membrane bound organelles
- Eukaryotic cells
- Produces a lot of ATP
- Mitochondria
- Human DNA contains _______ nucleotides
- 8 billion
- Function: Energy source, structural material
- Carbohydrates
- Somatic cells undergo _____
- mitosis
- Attaches pieces made by by Polymerase on lagging strand only
- DNA Ligase
- Guanine=
- Cytosine
- Function of DNA
- Information transmission
- Outward expression of a trait (appearance, behavior)
- phenotype
- Only eukaryotic cells have this organelle
- mitochondria
- Amino acids are the monomer of_____
- proteins
- Cell looks like it has a belt, maximum condensation, start to see chromatids (x)
- Metaphase
- When you see the heterozygotes (white + red = pink, not red)
- incomplete dominance
- Have no nucleus, no membrane bound organells (except ribosomes)
- Prokaryotic cells
- Single sub-unit
- monomer
- The division of a parent cell into two daughter cells
- Cellular reproduction
- Made of amine group (-NH2), a carboxyl group (COOH), A hydrogen, a variable group (R)
- Proteins
- Used by most organisms to produce amino acids
- DNA
- Tendency for a water surface to resist breaking
- surface tension
- Adenine=
- Thymine
- Who is the father of modern genetics
- Johann Gregor Mendel
- Alleles arise from _____
- mutation
- Thin barrier separating inside of cell (cytoplasm) from the outside environment
- Plasma membrane
- tRNA has 3 exposed bases that form base pairs with the mRNA codon
- anticodon
- Our body cannot make it, we must eat it
- essential amino acids
- Replication of cells in germ cells
- meiosis
- 2 types of nucleic acids
- DNA, RNA
- Molecules (proteins) that catalize chemical reactions
- Enzymes
- Fluid containing dissolved substances
- solution
- Process that forms and breaks chemical bonds holding molecules together
- chemical reaction
- What are the phases of mitosis?
- Prophase, Metaphase, Anaphase, Telophase
- Amino end of polypeptide always has the _____ atom
- nitrogen
- Stores, sorts and transports proteins and other molecules within the cell
- Golgi apparatus
- Transcription takes place in the _____
- nucleus
- Central Dogma of Biology
- DNA (transcription in nucleus) --> RNA (Translation in cytoplasm) --> Protein
- Homologous chromosomes that have different alleles for a gene
- Heterozygous ("different pair")
- Molecules that speed up chemical reactions
- Catalysts
- Alternate forms of a given gene (e.g. eye color)
- Allele
- Importance of water: water moderates temperature changes
- heats slowly, effective coolant, freezes slowly, forms ice
- RNA structure differs from DNA structure in 3 aspects:
- Single-stranded, ribose sugar backbone (dna=deoxyribose), uracil instead of thymine (A --> U)
- Discovered double helix of DNA
- Rosalind Franklin and Maurice Wilkens
- Species who have diverged and no longer interbreed
- Speciation
- Carrier =
- heterozygous individual (not affected)
- Oils and fats, waxes
- Lipids
- Joins nucleotide sub-units to form new strands of DNA, proof reading and dna repair
- DNA Polymerase (only goes in one direction)
- The energy source that directly powers cellular work
- ATP
- Specific location of a gene on a chromosome
- Locus
- Sister chromatids separate (mitosis revisited)
- Meiosis II
- Replication of cells in living things except germ cells
- mitosis
- How does information travel from DNA to Ribosomes?
- RNA (Ribonucleic Acid)
- All macromolecules have this element as a component (65% of human body)
- oxygen
- Start codon
- AUG (Methionine)
- Meiosis is similar to mitosis except the cell goes through __ nulcear divisions
- 2
- Movement of molecules from an area of high concentration to an area of low concentration
- diffusion
- Diagrams which show the genetic relationship among blood-related individuals (can reveal whether trait is dominant, recessive, or sex-linked)
- Pedigrees
- Place where proteins are created
- rough endoplasmic reticulum
- Find gene(s) of interest in DNA Libbrary ("clone" gene); Look for DNA Probe: short sequence of DNA that can form base pairs with DNA of interest
- Genetic Engineering Step 2
- Structural material: Chitin (exoskeleton- insects/crabs/spiders, fungus cell walls)
- Carbohydrates
- Specialized plasmids (bacteria) and viruses
- vector
- % of living organsisms that is water
- 60-90
- Plants resistant to disease (potatoes), plants that grow faster (poplar trees), animals that grow bigger (salmon)
- Applications of genetic engineering
- Kazutoshi Kobayashi wants to clone this
- wooly mammoth
- mRNA binds to ribosomes (protein factories) in cytoplasm
- Translation Step 2
- commercial use of alteration of biological materials to achieve specific, applied goals
- biotechnology
- Functions: catalyze chemical reactions, structure, energy storage, transport, movement, hormones, poisons
- Proteins
- Double-stranded double helix held together by ______ bonds
- hydrogen
- Translation continues until ____ _____ is reached
- stop codon
- Directs transportation of proteins and other molecules to the outside of the cell
- Golgi apparatus
- Enzyme activity is regulated by product concentration
- Feedback inhibition
- Made by exploiting natural means of recombining DNA
- Recombinant DNA
- Unreplicated chromosomes =
- 1 chromosome, 0 chromatids, 1 dna molecule
- Thymine=
- Adenine
- Human body is ___% water
- 65
- Composed of 1 Glycerol (a sugar alcohol) and 3 fatty acids (triglycerides)
- Lipids
- Molecules compete for the active site of the enzyme (usually not natural)
- Competative Inhibition
- Organisms which express genes that have been modified/ transplanted from other species
- transgenic
- Some cells never leave stage G1; they go to ____ phase
- G0
- Attachment site on chromosome for spindle fibers (at centromere)- late prophase, metaphase
- Kinetochores
- DNA --> RNA
- Transcription
- Made by the golgi apparatus; Transport food from cell membrane to other parts of the cell
- vacuoles
- A substance made up of different types of atoms
- compound
- Enzymes are synthesized in ____ form and activated only when needed
- inactive
- Interaction of 2 or more genes contribute to a single phenotype
- Polygenic Inheritance
- Division of cytoplasm
- cytokinesis
- XO (female) =
- Turner syndrome (infertile, often male-like)
- A particle composed of 2 or more atoms held together by chemical bonds
- Molecules
- This creates trait variation in populations
- Mutations
- Translation takes place in the ______
- cytoplasm
- Daughter cells are always separate in _____ cells
- animal
- Nucleotides joined via dehydration reaction
- Polymerization of nucleic acids
- Non-coding region of gene (RNA Polymerase binds to this)
- Promoter
- Proteins always have this element
- Nitrogen (only one!)
- Combines with protein to form ribosomes
- Ribosomal RNA (rRNA)
- Stored Energy (chemical bonds, electrical, positional)
- Potential Energy
- Cytosine=
- Guanine
- Amino Acid codes (Meth, Lys) are based on _____, not ______
- mRNA, tRNA Anticodon
- These form a ring around cell equator and contract to pinch cytoplasm into two parts
- Microfiliments
- Factors affecting hydrogen bonding in enzymes
- pH (optimal 6-8), salt concentration, temperature
- Area where DNA is in Prokaryotic cells, although it mostly floats around
- Nucleoid
- Neutral pH
- 7
- Hydrophilic head, hydrophobic tail
- Phospholipids
- Protein factories for cells
- Ribosomes
- RNA Polymerase binds to promoter region
- Transcription Step 1 (Initiation)
- Energy of movement (light, heat, electricity)
- Kinetic Energy
- The capacity to do work
- Energy
- Transcription produces a single strand of ____ that is complementary to one strand of ____
- RNA, DNA
- Made by the golgi apparatus; digest waste particles and malfunctioning organelles; highly acidic environments
- Lysosomes
- One or more nucleotide pairs are deleted from a gene
- Deletion Mutation
- Function: structural material, catalize cell processes
- Proteins
- Cylindrical structures, found in animal cells, that are composed of groupings of microtubules arranged in a 9 + 3 pattern. They help to organize the assembly of microtubules during cell division. (found in all phases)
- Centrioles
- Molecules electrically attracted to water
- hydrophilic
- Single-ring bases in DNA
- Pyrimidines
- Sex chromosomes are not the same size; X = ___x bigger than Y
- 10
- Movement of water from an area of high concentration of WATER to area of low concentration of WATER across semi-permiable membrane
- Osmosis
- How Alba: the green glowing bunny was genetically altered
- Green Fluorescent protein (GFP gene was extracted from a jellyfish)
- Daughter cells are always connected in _____ cells
- plant
- Entire set of chromosomes from a single cell
- karyotype (tend to be unique to a specie)
- Scientists want to bring this animal back that went extinct in 1936
- Tasmanian wolf
- Work with GFP
- Tracking cancer, finding malaria
- Mice where a gene is disrupted, so it will no longer produce the protein; enables researchers to determine the importance of the protein to the life of the organism
- knockout mice
- Genes encodes the amino acid sequence of a _______
- protein
- Fully express alleles on X chromosomes in _____
- males (genes expressed on the sex chromosomes, for males, will be expressed fully and will be passed down to all sons)
- Carboxyl end of polypeptide always has the ______ atom
- carbon
- One parent is always ______ ______ in test crossing
- homozygous recessive
- A skin cancer predisposition disease (genetic defects in the enzyme the repairs sunlight damage)
- xeroderma pigmentosum
- 4 bases combine in _____ codes
- triplet
- groups of similar cells with a similar function
- tissues
- Cell cycle
- Interphase, mitosis
- Nondisjunction with autosomal chromosomes don't usually survive to birth, unless it's chromosome __ (down syndrome)
- 21
- DNA is made up of:
- Deoxyribose (the sugar), Phosphate, nitrogen rich base (nucleotide; rungs of dna)
- Homologous chromosomes that have the same allele for a gene
- homozygous ("same pair")
- Amino acid polymers=
- polypeptides
- Since proof-reading enzymes correct mistakes made by polymerase, overall mistake rate = __ bp/ ______ bps
- 1, 1,000,000,000
- Composed of water-soluble sugar molecules
- Carbohydrates
- Small molecules bind to enzyme, changing shape of active site
- Allosteric Regulation
- Daughter cells contain 1/2 the genetic information of parental cell in _____
- meiosis (sexual reproduction- produces gametes)
- Collection of bacteria containing entire genome of organism in small pieces
- DNA library
- Genetic material, aka the hereditary blueprint
- DNA
- Turns enzyme on/off when needed
- Allosteric Regulatory site
- Daughter cells genetically identical to parent cells in _____
- mitosis
- Similar in structure to fats/oils except 1 of 3 fatty acids are replaced by phosphate group
- Phospholipids
- Form at opposite poles of the cell (prophase), extend from the cell poles to align chromosomes at the metaphase plate (metaphase), and pull the chromatids toward the spindle poles, and lengthen and elongate the cell (anaphase)
- Spindle Fibers
- All living organisms have ___ as their genetic material
- DNA
- Introduce gene into new organism (via plasmid, virus, or blast dna-coated pellets into cell 'gene gun')
- Genetic Engineering Step 3
- All other chromosome pairs other than sex chromosomes
- autosomes
- Cells ith only one of each type of chromosome (n)
- Haploid (found in sex cells)
- Cells in G0 phase still do their jobs (metabolically active), they just don't _____
- divide
- Change in gene frequencies in a population over time
- evolution
- Number of protons in the nucleus
- Atomic number
- __________ are polymers of aminoasicds
- Polypeptides
- Resting, growing phase
- Interphase
- All sexual life cycles include 2 events:
- Meiosis, fertilization
- G1 Phase
- Growth phase 1 (acquire materials, grow)
- Chromatids have been ripped apart, 92 chromosomes, DNA has been divided
- Anaphase
- Double-ring bases in DNA
- Purines
- Single circular chromosome attached to cell wall
- Prokaryote DNA
- Locations of the organism's genome (DNA)
- Nucleus
- Bacteria, Archaea
- Prokaryotic cells
- Phospholipids, Steroids
- Lipids
- Mitosis or Meiosis only occurs in _____ cells
- Eukaryotic
- Can carry info, but cannot store it
- RNA
- Zygote
- fertilized egg (1-cell embryo)
- Late anaphase - early telophase
- cytokenisis
- Molecules electrically neutral, and therefore not attracted to water
- hydrophobic
- The human Y chromosome carries __ genes
- 84
- Binary fission (a form of mitosis) only occurs in ______ cells
- Prokaryotic
- XYY (male) =
- Fertile; excess testosterone; lower IQ)
- Human karyotypes tend to order chromosome pairs from _____ --> _____
- longest, smallest
- Lipid production, including steroids; in liver cells, it detoxifies drugs and alcohol; also in liver cells, it breaks down glycogen
- Smooth endoplasmic reticulum
- Functions of Mitosis
- Growth, Maintenance, Repair, Asexual reproduction
- Replicated chromosomes =
- 1 chromosome, 2 chromatids, 2 dna molecules
- Last step in PCR, once enough dna is made
- compare segments with suspects (victims, and other people involved too)
- 4 rings of carbon with functional groups attached
- Steroids
- pure liquid inside of cell
- cytosol
- Sister chromatids connected at ______
- centromere
- RNA always has ________ as sugar
- ribose
- Genetically modified organisms (part 2)
- BT Corn, Flavr Savr tomatoes (A gene was inserted to make ripe tomatoes firmer, since normal tomatoes cannot be shipped when ripe), golden rice (modified to make vitamin A by using a daffoldil gene; still not enough to make a difference)
- Enzymes are all _____
- Proteins
- Long chain of monomers
- polymer
- Region where paired chromosomes are joined
- Chiasmata
- Breaks hydrogen bonds holding nitrogen bases together (unzips)
- DNA Helicase
- This starts the moment the RNA leaves the nucleus
- Translation
- Chromosomes often occur in paird called ______
- homologues
- Structural Material: cellulose (plants- composes cell wall), not digestible by most animals
- Carbohydrates
- Each triplet stands for an _____ _____
- amino acid
- RNA --> Protein
- Translation
- The modification of genetic material
- genetic engineering
- tRNA delivers appropriate amino acid to ribosome
- Translation Step 3
- Batteries of the cell
- mitochondria
- Only one strand of DNA (template strand) is _____
- copied
- Warehouse of the cell
- golgi apparatus
- Polypeptide backbone
- NCCNCCNCC
- Semi-liquid
- cytoplasm
- G2 Phase
- Growth phase 2 (synthesize material for cell division)
- DNA always has ________ as sugar
- deoxyribose
- The human X chromosome carries ___ genes
- over 1500 (close to 2000 now)
- Main component of the plasma membrane
- Phospholipids
- Most common energy-carrier molcules; synthesized from ADP
- ATP
- Formation of haploid gametes to diploids (1n + 1n --> 2n)
- Fertilization
- Cells are...
- the smallest unit of life
- S Phase
- Synthesis phase (replicate DNA)
- A molecule upon which an enzyme acts; binds with the enzyme's active site
- Substrate
- Three bases are called _______
- codons
- Splitting of diploid to haploid gametes (2n --> 1n)
- Meiosis
- Crossing a dominant phenotype individual (unknown if they are Pp or PP) with a homozygous recessive individual to determine what the genotype of the dominate individual
- Test cross
- Chromosome replicates, cell elongates (chromosomes pulled apart), membrane pinches in middle, daughter cells formed
- Binary Fission
- Genetically modified organisms (GMO's)
- Fishberry (strawberry with flounder genes for antifreeze proteins), Frostban (bacteria with one gene altered; plants sprayed with bacteria have less frost damage)
- XXY (male) =
- Klinefelter Syndrome (Infertile; often unnoticed)
- Liquid capable of dissolving other substances in itself
- solvent
- Binds other part of tRNA and has enzymatic site where amino acids are linked together
- Large Ribosomal Sub-unit
- This is the main component of organic molecules
- Carbon
- Stop codons
- UAG, UAA, UGA
- Function: Energy storage, structural material
- Lipids
- The process when environmental differences results in changes of the gene frequencies in a population of organisms
- natural selection
- Cells with pairs of homologous chromosomes (2n)
- Diploid (found in most cells of human body)
- XXX (female) =
- Trisomy X (Fertile; usually tall; potentially lower IQ)
- cells are created by cell _____
- replication
- Base pair rules apply in Transcription except that _______ replaces ______
- uracil, thymine
- Chromosomes have condensed (no chromatids yet)
- Prophase
- 23-year-old man, killed by blow to face, lived ~9000 years ago
- Cheddar man (mtDNA shows relationship to several living descendants in nearby village)
- mRNA carries code for protein from nucleus to cytoplasm (Exits nucleus via nuclear pores)
- Translation Step 1
- smallest living unit
- cell
- Crossing over occus during _____ of meiosis I
- prophase
- When Homologous pairs don't separate during meiosis (affects gamete chromosome counts)
- Nondisjunction
- Water is an excellent ______
- solvent (important!)
- RNA Polymerase "forces" DNA to partially unwind, RNA Polymerase synthesizes complementary copy (only frees up one gene that will be made into protein)
- Transcription Step 2 (elongation)
- Polypeptides=
- Proteins
- Long hydrocarbon skeleton, terminal carboxyl group
- Fatty Acids
- Carries code from DNA to ribosomes
- Messenger RNA (mRNA)
- Plasma membrane
- phospholipid bilayer
- Location on enzymes where substrates can bind
- Active site
- Chromosomes that undergo crossing over are known as _____ chromosomes
- recombinant
- True-breeding =
- homozygous
- Once enough dna is made using PCR, they are cut with _______ _______
- restriction enzymes (these enzymes are made naturally by bacteria to "chop up" virus DNA)
- DNA Polymerase mismatches __ bp/ _____ bps
- 1, 10,000
- Small sized cell with nucleoid
- Prokaryotic cells
- Majority of cell cycle is spent in _______
- interphase
- RNA Polymerase reaches termination signal, RNA Polymerase detaches from DNA strand, RNA molecule detaches from RNA Polymerase, DNA zips back up
- Transcription Step 3 (Termination)
- 5-carbon sugar (ribose or deoxyribose), phosphate group, nitrogen-containing base (5 types)
- nucleotides
- Prepare Recombinant DNA; restriction enzymes cut up large dna molecules into smaller pieces (location of cut is based on specific dna sequence); Insert fragments into vectors (transform recombinant vectors into bacteria; bacteria randomly pick up vector)
- Genetic Engineering Step 1
- Monosaccaride, Disaccharides, polysaccharides
- Carbohydrates
- All organic matter has this element
- carbon
- Needed for structural integrity of cell membrane, absorption of vital vitamins
- Cholesterol
- Molecules composed of nucleotides
- Nucleic Acids
- Binds mRNA and part of tRNA
- Small Ribosomal Sub-unit
- 2 types: free, and attached to the endoplasmic reticulum
- Ribosomes
- A pair of bases is incorrectly matched
- Point Mutation
- Function in Mammals: Store tissures in adipose cells, cushioning and insulation
- Lipids
- Sugar-phosphate backbone
- Nucleic acids
- Rough and smooth organelle
- Endoplasmic reticulum
- One or more nucleotide pairs are inserted into a gene
- Insertion Mutation
- Function: Hormones (including sex hormones: estrogen, testosterone and cortisol: stress hormone)
- Lipids
- Substance that can't be broken down or converted to another, simpler substance by ordinary chemical means
- element
- DNA Strand being transcribed
- Template Strand
- Exchange of segments of homologous chromosomes
- Crossing over