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CSF

CSF organisms for Microbiology lab

Terms

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CAMP, Hippurate, Bacitracin, PYR
The four identifying tests for GBS.
Haemophilus influenzae
Smells like a Micro lab hallway.
Listeria
Grows well on BAP and looks like GBS with small zone of hemolysis excepting that the catalase result is positive as opposed to the GBS.
14-45 mg/dL
Normal protein level.
Neisseria meningitidis
Gram negative diplococci that look like kidney beans.
Listeria
Likes cold temperatures and refrigeration, and is found often in dairy products during outbreaks
alpha-hemolysis
Streptococcus pneumoniae shows this kind of hemolysis.
<5 lymphs/mL
Normal lymphocyte count for CSF.
positive, positive
Haemophilus influenzae is catalase:____________ and oxidase:____________.
GBS
This organism is responsible for childbed fever and related fatalities in women and babies.
Porphobilinogen or porphyrin
What is produced by an X factor independent organism in the Porphyrin test?
Haemophilus influenzae
Gram negative coccobacillary rod that is hard to see on a gram stain, but can be found in RBCs.
Capnophilic
Incubation in CO₂.
Heat
What allows Haemophilus to grow on sheep's blood agar?
X Factor
Heat stable tetrapyrrole compounds that are found often in iron-containing pigments.
Viridans strep
Other alpha streps are called this...
Fluorescence
A positive result in the porphyrin test is...
BAP, CHOC, and Thayer Martin
Agars that Neisseria grows well on.
57%
S. pneumoniae urine test accuracy.
Optochin sensitivity
This test requires CO₂ incubation and shows sensitivity at >14mm of zone.
positive, positive
Neisseria meningitidis is catalase:____________ and oxidase:____________.
Beta-lactamase
Always do this test if Neisseria meningitidis is discovered.
Viral meningitis
An increase in lymphocytes (200-500), normal glucose, and slightly elevated protein levels.
Listeria
Gram positive rod that is a facultative anaerobe that is non-sporeforming.
GBS
Gram positive cocci that are in pairs and short chains and have large colonies and small zones of beta-hemolysis.
Porphyrin test
This test looks for X factor independent Haemophilus.
Bile solubility
This test looks for the presence of autocatalytic enzymes in S. pneumoniae.
Bacterial meningitis
An increase in neutrophils (500-20,000), decreased glucose, and elevated protein levels.
45-100 mg/dL
Normal glucose value.
V Factor
NAD or NADP

Deck Info

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