Mirco Lab final

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What are the reading criteria fpr BA (4)? (Name types of growth): (1)no growth evident (2)Beta hemolysis (3)Alpha Hemolysis (4)Gamma hemoylsis
If the media is BA and there is no growth, what is the microbe?: cant tell bc no growth
If the media is BA and there is clear zone around the colony, what is it?: Beta Hemolysis
IF the media is BA, and the culture has green decolorazation from the top, what is it?: Apha hemolysis
If the media is BA and the culture has no color change in the medium, what is it?: Gamma hemolysis
What is gamma hemolysis?: is in BA and has no color change
What is Alpha hemolysis?: is in BA and has green decolorization at the top
What is Beta hemolysis?: is found in BA and has a clear zone around the colony
What are the reading critreia for MAC? (what organimsms can it be) (2): (1)fermenter (2)nonlactose fermenter
What is a fermenter? (2): is found in MAC and is pink and red
What is nonlactose fermentor?: is found in MAC and is colorless
If the media is MAC, and it is colorless, what is it?: nonlactose fermentor
If the media is MAC, and its color is Pink or red, what is it?: fermentor
SA stands for (1): Salt agar
What are some ph incolulators? (3): (1)TALU (2)TSI (3)UB
(1) is a ph indicator: tMSA
tMSA is a (1): ph indicator
when is the ph postive?: when its is phenol red
What is the reading criteria for MSA? (3) (name organisms it can be): (1)Staphylococcus aereus (2) streptococcus epdidermis (3)Staphloycoccus
when is a organism stapholycoccus aereus?: if it is in MSA media and if medium growth turns a lemon yellow
If the media is MSA and the medium growth turned a lemon yellow, What is it?: Stapholyoccous aereus
When is the culture Streptococcus epidermis?: if it is in MSA media and the growth medium is pink
If the media is MSA and the growth medium is pink, what is the organism?: Streptococcus epidermis
When is the organism stapholyoccus?: if the media is MSA and there is no growth
If the media is MSA and there is no growth. what is the organism?: stapoholyococcus
what organisms can be found in the medium PA ? (2): (1)Pseudomonas (+)
(2)Pseudomans (-)
When is a culture pseudomanos (+)?: if the media is PA and the growth is streaked on a line
What is a culture psedumonas (-): if the media is PA and there is no growth
If the media is PA and there is no growth. what is the organism?: pseudomonas (-)
if the media is PA, and there is growth streaked in a line, what is the media?: Pseudmonas (+)
What does CIT stand for?: Simmon's Citrate agar
What does SIM stand for?: Sulfide Indole Motility Medium
What does TSI stand for?: triple Sugar Iron
What is the purpose of CIT?: dectection of citrate utilizers
NAME purpose is the decection of citrate utilizers: CIT
What is catergory of CIT?: biochemical
What is the catergory of SIM?: biochemical
What is the catergory of TSI?: biochemical
What is the catergory of UB?: biochemical
What medias are under the caterogry biochemical? (4): (1)CIT (2)SIM (3)TSI (4)UB
What is the purpose of SIM?: screening for indole and H2S production and motility
NAME purpose is the sceening for indole and H2S production and motality: SIM
What is the purpsoe of TSI?: screening for fermentative ability
NAME purpose is for the screening of fermenative ability: TSI
What is the purpose of UB?: producation of exoenzyme urease
NAME purpose is the production of a exoexnzyme urease: UB
What does UB stand for?: Urea Broth
If the culture is in a UB media, what organisms can be found? (2): (1)urease (+) (2)urease (-)
When is the culture urease (+)?: if it is in the media UB and is fuschia
when is the culture urease (+): if the media is UB and a fuchsia color
IF the media is UB and the culture is fuchsia color, what is the organism?: urease (+)
if the media is UB, and the culture is a orange color, what is the media?: urease (-)
What is the purpose of UV radiation in a experiment? and how is it limited?: (1)has a lethal effect on bacteria and can therefore be used as a sterilzing agent (2)bc bacteria can repair uv damage
UV light can cause the (1): formation of thymine dimers
What are enzyme is used in the light repair?: DNA photoylase
What enzymes are used in dark repair?: (1)helicase (2)DNA polymerase (3)DNA Ligase (4) endonuclease
What is the function of helicase in dark repair?: removes nucleotides
What is the function of endonuclease in dark repair?: detects the thymine dimer
What is the function of DNA polymerase in dark repair?: inserts complementray DNA nucleotides
What is the function of ligase in dark repair?: seals the sugar phosphate bone
What is the function of DNA photolyase?: a repair enzyme that reversese the reaction
E coli can perform (1)repair: both light and dark
What is the kirby and baver test?: a culture and senstivity test that measures the zone of inhibition
NAME is a culture and senstivity test that measures the zone of inhibition: kirby and baver test
NAME is called photoreaction: light repair
NAME is called excision repair: dark repair
What is another name for dark repair?: excision repair
What does STA stand for?: Snyder's Test Agar
What is the purpose of STA?: dectect and ID lactobacilli
What is the catergory for STA?: Selective and differintial
NAME the purpose of this media is to dectect and ID lactobacilli: STA
What is STA ph indicator?: bromcresol green
Bromcresol green in (1) ph indicator: STA
What does LSB stand for?: Lauryl Sulfate broth
what is the purpose of LSB?: detect and ID coliforms in food
NAME the purpose of this media is to detect and ID coliforms in food: LSB
What caterogty is LSB ?: differential and selective
How do you tell what microbe it is if it is a LSB media?: (1)if there is a gas buble in the durham's tube then it is "coliforms +" (2)if there is no gas then it is coliforms - "
If the microbe is in a LSB media, and has a gas bubble in the Durham's tube, what is it?: coliforms +
If the microbe is in a LSB media, and has no gas bubbles, what is it?: coliforms -
NAME MEDIA the microbe was found to be coliform +: LSB
What is the bacterial lawn?: space around the anitbiotics
What does BEA stand for?: Bile Escular Agar
What is BEA's catergory?: Selective and differential
What does EMB stand for?: Eiosan metheylene Blue
NAME is a "fecal contmination indicator": E coli
NAME has a red pigmentation and causes pneumonia in alcholics and in wounds of burn patients: Serrshatia marcesens
What is transformation?: when live bacteria absorbs DNA fragments and expressises new traits
What is serrshatia marcescens? (2): (1)has red pigmentation (2)causes pneumonia in alcholics and in the wounds of burn patients
What is the purpose for EMB?: to detect califorms which detect fecal contamination
If it is in a UB media and is a orange color, What is it?: urease -
If it is in UB media and is fuschia color, What is it?: urease +
If it is in CIT media and is a Blue color, What is it?: Citrate +
If the media is SIM and it is a black color, what is it?: H2S+
If the medida is CIT and the media is green, What is it?: Citrate -
If the media is SIM, and it is cloudy, What can you conclude?: moltitly+
If the media is SIM, and you add (1) and turns red, What is its readings?: (1)Kovac (2)idole +
If the media is TSI, and the slant is red,, What is it?: K for alakaline
If the media is TSI, the slant is yellow, What are the readings?: A for acid
If the media is TSI and the butt is red, what is the readings?: K
If the media is TSI and the butt is yellow or black, what are the readings?: A
If the media is TSI, the slant is red, the butt is yellow, and there is no gas production, what are the readings?: (1)AK+ (2)only glucose is fermented
In TSI, when is there only glucose fermented?: red=slant and yellow butt
In TSI, when is there Glucose and lactose fermented and or sucrease feremented?: yellow slant and butt
How do you tell if there is no H2S production?: no black
What is the Blood order?: Never Let Monkeys Eat Bannanas

N=neutrophillis
L=Lymphocytes
M=monocyetes
E=Eosoisinophilis
B=Basophilis
If a person has a fungal infection, what are the max types of blood cells present?: Esoisinohilis
If a person has a infection caused by a Helimenth, what are the max types of blood cells present?: Esuisinophillis
If a person has increased levels of histermenes resulting in incresased allergies, what are the max types of blood cells present?: Basohilis
If a patient has increased levels of Basohilis blood cells, what do they likely have?: allergies and increased levels of histomenes
If a patient has increased levels of monocytes, what do they likly have?: is non-specific nad increase w any infection
NAME these blood cells increase when a person has a infection: monocytes
If a patient has increased levels of Esosinophilis blood cells, what do they likely have?: when you have a fungal infection by Hemimeths
If a patient has increased levels of Neutrophilis in their blood, What do they likely have?: bacterial infection
A patient has a bacterial infection, what are the max types of blood cells present?: Neutrophilis
If a patient has increased levels of lymphocytes, what do they likely have?: a viral infection
A patient has a viral infection, what are the max types of blood cells present?: lymphocytes
What is Blood types A? (4): (1)has Anigen A (2)the Anitbody in plasma is Anti-B (3)Rxt w Anti A =+(4)Rxt w AntiB= -
What is Blood type B?(4): (1)has antigen B (2)Anitbody in the plasma is anti A (3)Rxt w Anti B = - (4) Rxt w Anti B=+
What is Blood types AB? (4): (1)has antigens A and B (2)is not anti anything (3)Rxt w Anti A = + (4) Rxt w Anti-B=-/+
What is blood type O?: (1)has nether A or B antigens (2)Rxt w Anti-A= - (3)Rxt w Anti-B=-
for the blood types, which ones are domminant and recessive of the O, A, and B?: A and B are co-dominant and O is recessive
Which medias are used for unkown projects?: (1)MAC (2)CIT (3)GLU (4)SIM (5)UB (6)TSI
Which medias are used for food?: (1)EMB (2)LSB (3)MSA (4)PA (5)TSA
Which media is used to test for dental carries?: STA
NAME category is general: TSA
NAME category is Selective and Differiental (4): (1)EMB (2)MAC (3)MSA (4)STA
NAME category is biochemical (7): (1)CIT (2)GLU (3)SIM (4)UB (5)TSI (6)LSB (7)PA
What are the specail ingredents in MSA?: salt
Which media's contain Durham tubes? (2): (1)GLU (2)LSB
Which medias have a low ph?: STA
Which medias are pH indicators?: (1)UB, TSI, and MSA= phenol red (2)Bromcresol green=STA
What is the pH indicator for STA?: bromcresol green
What is the pH indicator for UB, TSI, and MSA?: phenol red
NAME media is a respiration/ fermentation test: TSI
TSI is a (1) test: respiration/fermentation
NAME media is used to test milk quailty: Methylene Blue Reductase test
What is Methylene Blue Reducatse test used to do?: test the quaility of milk
For the Methylene Blue Reductase test, how do you tell if the milk is good or not?: (1)if it changed from blue to white in under two hours= poor quailty of milk (2) if changed after 6 hours=good quaility
For the Methylene Blue Reductase Test, blue=? and white=?: (1)oxidized (2)reduced
What is the media LSB and ESB used for?: to find fecal contamination
What is the media MSA used for?: handling
What is the media PA used for?: spoilage of water
What is the media TSA used for?: general bacteria count in food
NAME MEDIA is used to find fecal contamination? (2): LSB and ESB
NAME MEDIA is used to test foods for handling: MSA
NAME MEDIA is used to count baceteria in the food: TSA
What media where used to test Iced Tea?: (1)EMB (2)LSB (3)PA (4)TSA
EMB, LSB, PA, and TSA used for??: to test iced tea
Why was not MSA used to test iced Tea?: bc it contains tannic acid which would interact w salts and give you a fasle postive
In the transformation experiment, why did we include ampiciillin in the medium?: to show the transformed cells
NAME is antibiotic senstivity test: Kirby-Bauer test
What does aggulination mean?: clumping
what increased pathological smear of mono blood ?: Basisphis
What reagent is required for the reading of SIM?: Kovacs
What are the readings for CIT?: (1)Blue= citrate +
(2)Green = citrate -
What are the readings for SIM? (3): (1)black=H2S+ (2)Cloudniss at inculation line=moltitly + (3)when Kalvoc is added and it turns red it is indole +
What are the readings for GLU?: (1)Red= K or yellow = A (2) gas = + or -
What are the readings for MAC?: (1)Pink/red= fermenator
(2)colorless= nonlactose fermenator
What is the purpose of BA?: test for hemolytic acitivty
NAME purpose is to test for hemolytic activity: BA
What are the readings for MSA>: (1)S. aureous =yellow/lemon
(2)pink= s. epidermis
(3)no growth = stapholycoys -
What is the purpose of MAC?: isolate gram - enterics
NAME purpose to isolate gram - enterics: MAC
What is the purpose of MSA?: isolte Staph species
NAME purpose is to isolate staph species: MSA

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