biochemtest3-ligandbinding-SPR
Terms
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- What are advantages of SPR
- real-time, unique-high quality data, simple assay, small amount of sample only needed
- plasmon (2)
- a hybridization of conducting electrons and photons
- plasmon
- oscillation of the conduction electrons in a metal.
- magic angle
- total internal reflection
- What phenomenon occurs with SPR?
- electron charge density wave
- What information can the sensorgram tell you?
- association, concentration, kinetics, dissociation, regeneration
- What are the three parts to SPR?
- 1. Biacore sensor chip 2. Integrated microfluid cartridges 3. Surface plasmon resonance detection
- Kinetics is need for finding:
- structural changes, characterization of biopharmacuetical products, assays based on affinity, purification schemes
- SPR
- Surface Plasmon Resonance
- How is binding constant determined with SPR
- bait ligand coated to gold surface of SPR crystal, prey ligand flows over bait layer and binds, SPR signal changes to new equilibrium, solution without prey is applied, new equilibrium reached. The Voff and Von can be used to calculate the binding constant.
- Energy and momentum are transformed to
- incident photons into surface plasmons
- Quantitative measurments at the Biacore sensor chip are dependent on:
- the immobilization of a target molecule to the sensor chip surface
- SPR affected by
- coupling of the gold layer. Binding of bait and prey affects the total internal reflection angle change
- SPR can tell you
- yes/no data, how much, how strong, how fast
- Resonance is due to
- energy and momentum