VCL lab practical
Terms
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- What is the purpose of measuring the PCV?
- To tell if the animal is anemic or not and to determine the percent of RBCs circulating in the peripheral blood at the time of collection
- List three common measurements of the red blood cells
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PVC - %
Total protein- g/dl
Unopette- RBC/mm^3 - Lipemia
- Fat in the blood - has a cloudy pinkish color
- Hemolysis
- red/pink color
- Icterus
- Yellowish color, liver problems
- When performing a WBC count and/or a platlet count why is it recommended to let the unopette sit for 10 minutes prior to charging the hemacytometer?
- This allows the red blood cells to hemolyze
- On a hemacytometer where is the leukocyte count performed?
- in all 9 the the big squares on the grid (both sides)
- On a hemacytometer where is the platelet count performed?
- only on the middle square of the grid (all 25 boxes)
- What diluent is used in the BD Unopette system for the determination of leukocytes and plalets? What role does the diluent play?
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Ammonium oxalate
it lyses the blood cells - What diluent is used in the BD Unopette system for the determination of leukocytes? What role does the diluent play?
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Acetic acid
it lyses the blood cells - What precautions must be taken with the Diff Quick stain set?
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~ covers on at all times: evaporate, doesnt spill, or become contaiminated
~ Use them in the correct order - Poikilocytosis
- Abnormally shaped erythrocytes
- Anysocytosis
- The RBCs are irregular with a few, unevenly distributed surface projections
- Rouleau formation
- the grouping or erythrocytes in a stack
- Aggulutination
- A grouping of erythrocytes involving the bridging or clumping of RBCs - occurs in an immune-mediated disaorder
- Why do we avoid evaluating the blood smear at the feathered edge
- The cells are too spread apart and also may be broken into fragments and dont correctly represen the blood
- What happens to the blood cells when we heat the slide up the drying process?
- The cells are destroyed
- Will a reticulocyte eventually lose its reticukum? why?
- Yes the reticulocyte will lose its reticukum because within a few days it will mature into a RBC and no longer need the reticulum
- Can one perform a reticulocyte cont on the blood of a horse? Why?
- No because they do not release reticulocytes from the bone marrow even in the face of anemia
- What is thrombocytopenia?
- The presence of relatively few platelets in blood
- What are 3 situations in which thrombocytopenia may occur in a dog or a cat?
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~decreased production
~peripheral destruction
~mediated induced - Neutrophil
- seeks out and kills microorganisms, ingests as well
- Band neutrophil
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similar to regular neutrophil but nucleus is horseshoe shaped
immature neutrophil - Lymphocyte
- work for immune system, respond to antigens
- Reactive lymphocytes
- immature lymphoid cells contain a nucleolus within the nucleus, usually surrounded by chromatin
- Eosinophils
- respond chemically to bacterial products and complement fragments, control parasitic infection
- Monocytes
- precursors of macrophages, role of imune function
- Basophils
- production of histamine and heparin
- Macrophages
- phagocytisis, antigen processing cells
- Define Passive immunity
- the immunity you receive from the colostrum of your mother or from vaccines. Not permanent.