Presumptive Bacterial IDs
Terms
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Highly mucoid, acid on Lemb/MAC
Spot indle neg - Klebsiella pneumoniae
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Highly mucoid, acid on Lemb/MAC
Spot indole pos - Klebsiella oxytoca
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Green sheen on LEMB
Oxidase neg
Spot indole pos - E.coli
- Swarming
- Proteus
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Metallic sheen on BA
Blue-green on LEMB
Grape odor - Pseudomonas aeruginosa
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Small, translucent, gray, shiny, beta-hemolytic.
Catalase neg; PYR pos; GPC - Strep group A
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Small, translucent, gray, shiny, gamma-hemolytic.
Catalase neg; PYR pos; GPC - Enterococcus
- What are the criteria for doing a presumptive ID?
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-Pure culture
-Highly experienced tech - What causes methicillin resistance?
- the Mec A gene
- What does the Mec A gene encode?
- Altered binding proteins
- What is the best method for Methicillin resistance testing?
- Oxacillin disc, 6 ug, 35'C for 48 hrs, 2% NaCl in Mueller Hinton Agar.
- What is better at curing methicillin resistant organisms?
- Combination of Vancomycin + Rifampin
- Whats problem re: Strep pneumo antibiotic susceptibility?
- Penicillin susceptibility
- What is the choice method for detecting Strep pneumo penicillin resistance?
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-1 ug Oxacillin disc
-Mueller Hinton + 5% Blood agar
-Increased CO2 - What are the ref ranges for Strep pneumo oxacillin -resist testing?
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If 20 mm or greater, SUSCEPTBLE.
If less than 20, RESISTANT.