Micro Lab 2

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BA stands for: Blood Agar
What catergory is BA under?: Differential
What is the purpose of BA?: to test for hemolytic acitvity
What does MAC stand for?: MacConkey Agar
What catergory is MAC under?: Differential and Selective
What is the purpose of MAC?: for isolation of Gram (-) Enterics
What does PEA stand for?: Phenylethyl Alcholol Agar
What catergory is PEA?: Selective
What is the puropse of PEA?: Isolation of Gram (+)
What does MSA stand for?: MAnnitol Salt Agar
What cateogry is MSA under?: Seletive and differential
What is the purpose of MSA?: Isolate spahtyloccus species
What does PA stand for?: Pseudomonas Isolation Agar
What cateogry is PA under?: Selective
What is the purpose of PA?: isolation of pseudomonas species
What is Staphylococcus epidermidis? (3): (1)on the skin (2)opportunistic (3) most strains produce a slime layer which may enalble them to attach to cetrain hopstial apparatuses during invasive procedures
NAME can be found on the skin and is opportunistic: Staphylococcus epidermidis
NAME most strains produce a slime layer which may enalble them to attach to cetrain hopstial apparatuses during invasive procedures: Staphylococcus epidermidis
What is Stahphylococcus aureus? (2): (1)most common noscomial bacteria infection (2)causes food poisning, toxic shock, scalded skin syndrome, pinke boils etc
causes food poisning, toxic shock, scalded skin syndrome, pinke boils etc: Stahphylococcus aureus
NAME is the most common bacteria nosocomial infection: Stahphylococcus aureus
NAME also known as GAS: Streptococcus pyogenes
GAS stands for: Group A Streptococcus
Streptococcus pyogenes are also called ( ).: GAS
NAME is the most common cause of pharyngitis, but can also cause acute rhematic fever, and acute glomerulonephritis: Streptococcus pyogenes
What is Streptococcus pyogenes? (3): (1)also called GAS (2)common cause of pharyngitis (3) can also cause acute rheumatic fever, and acute glomerrulonephritis
NAME is the most common cause of pharyngitis: Strepcoccus pygones
Pharyngitis is ( ).: Sore throat
Sore throat can also be refered to as ( ).: Pharyngitis
What is Pseudomonas aeruginoa? (4): (1)Opportunitstic w phagocyte resistant slime layer (2) has a fruity odor (3) a blue green pigment (4) can cause eye and ear infections
NAME Opportunitstic w phagocyte resistant slime layer: Pseudomonas aeruginosa
NAME is nosciminal and can cause eye and ear infections: Pseudomonas aeruginosa
NAME has a fruity odor and a blue green pigment: Pseudomonas aeruginosa
What is Escherichia coli?: (1)common resistant of digestive tract (2) produces Vitamin K and B (3)is a indicator bacterium
What can Escherichia coli cause(3)?: (1)food poisning (2) Montezumas revenge (3) UTI
NAME common resistant of digestive tract: Escherichia coli
NAME produces Vitamin K and B: Escherichia coli
NAME is a indicator bacterium: Escherichia coli
NAME usual is the cause of UTI, food poisning, and Montezuma's revenge: Escherichia coli
Describe the Mircococcus gensis (3): (1)Gram (+) (2)aerobic (3)nonpathogenic
What is Micrococcus Luteus?: appears yellow in culture
What is Micrococcus?: appears red in culture
NAME is gram (+), aerobic, and nonpathogenic: Micrococcus luteus and Micrococcus roseus
NAME appears yellow in culture: Micrococcus luteus
Are species of the genre Micrococcus pathogenic?: No
NAME appears red in culture: Micrococcus roseus
Where is the sharps container located?: in the sliver container at the end of each lab table
What is a nosocomial infection?: a hospital acquired infection
How do you label plates and tubes?: (1)Media (2)organism (3) Team (4)Date (5)Intials
Why do you label at the bottom of plates?: for safety reasonsn incase someone removes the top and it becomes lost
What is the differ btwn Antiseptic and Disinfectant?: Antiseptic kills all germs on living tissue and Disinfectant kills tissue on inmate objects
NAME kills all germs on living tissue: Antiseptic
NAME is a hopstial acquried infection: nosocomial infection
NAME kills tissue on intimate objects: Disinfectant
What is the general prepartation for tubes?: melt before pipetting and autoclaving
What is the general preparation for plates?: autoclave and then pour
For ( ). the general preparation involes melting before pipetting and autoclaving: tubes
For ( ), the general preparation involes melting before pipetting and autoclaving: tubes
NAME Category is General (2): (1)TSA (2)TSB
NAME Category is Selective: PA
NAME Category is Differential: BA
NAME Category is both Selective and Differential (2): (1)MSA (2)MAC
What are the main differnces btwn preparing for media for tubes and plates?: For the tubes-melt before pipetting and autoclaving.

For the plates-autoclave and then pour
NAME Category is Bichemical: TGB
What is the Category of TSB?: General
What is the category of TSA?: General
What is the catergory of TGB?: Biochemical
What are the steps in preparing a smear?: (1)a drop of water (2)add organism (3)smear to the size of a quarter (4)air dry (5)heat fix
Where does the contaminated Lab material go?: in the Biohazardous Waste Disposal
Give one difference btwn Antiseptic and Disinfictant with one ex of each: Antispetic technique is used to keep tissue free of microbes and a ex is sterilizing medical equipment (2)Disinfectant is to clean the surface are and a ex is disinfectant used to kill microbes when there is a spill during lab
What are the steps used to clean up a contaminated spill? (4): (1)put a paper towel over the spill (2)spray the paper towel w disinfectant (3) wait 20 min (4)clean the spill
Which method of sterlization is used to destroy endospores and what type of heat sterliation is used?: the autoclave is used at a temp of 121 C at 15 psi
If by any means while doing pratical your glass slide breaks where then where should u place it?: the sharps container
Which standard method is used in micro lab for preventing and avoiding contamination of cultures and who used it first?: (1)Joseph Lister (2) aseptic techinques such as washing your hands before surgury
What does read the meniscus mean?: read to the line lowest where the water appears to be
What does Tare the balance refer to?: making a 1 plus zero balance
A recipe has 30 grams/1 liters, and how many grams of 160 ml do you want.: 4.8 grams
How do you solve recipe problems?: convert the give grams to mL then mutilpy by the other given
A recipe has 50 grams/1 Liter, how many grams of 350 mL are needed?: 17.5 grams
What does BA stand for?: Blood Agar
What does TGB stand for?: Thioglycolate Broth
What does TSA stand for?: Trypitcase Soy Agar
What does TSB stand for?: Trypticase Soy Broth
What is the purpose of TGB?: growth of anaerobic microbes
What is the purpose of TSB?: the growth of a wide range of bacteria
What tools do you use to incolulte the media? (3): (1)loop (2)needle (3) swab
Name two ways for incolution of media: (1)Agar slant (2)Agar plate
What is Agar slant?: a liquid broth placed in deep agar tubes
What is agar plate?: is a quadrant streak used for isolation or seperation of a mixed culture
What is Clostridium sporogenes? (4): (1)anaerobic (2)needs a medium such as TGB (3) does not produce neurotoxins (4)can cause inststinal water loss and hemorrhaging by destroying instetinal epitheaial cells.
NAME are anaerobic and needs a medium such as TGB: Clostridium sporogenes
NAME does not produce neurotoxins: Clostridium sporogenes
NAME can cause intestinal water loss and hemorrhaging by destroying inestinal epitheal cells: Clostridium sporogenes
What are Bacillus megaterium? (3): (1)aerobic (2) produce endospores (3) easy study under the light microscope
NAME is areobic and produce endospores: Bacillus megaterium
NAME are easy to study under the light microscope: Bacillus megaterium
Name two ocular adjustments you have to make in order to avoid eye strain and headache associated w prolonged microscopic use: adust the inerpulpllliary distance and the diapter reading to 65 to 40
Why are microbes hard to see in wet preparations?: bc they are moving around, making it harder to focus on one microbe for a long period of time
How do you achieve subdued illumination when examining wet mounts?: you would darken the background by using the condenser and the illuminator
What organs of locomotion do you find in each of the living protozoans?: (1)pseudopods (2)cilla (3)flagella
Why do you need to use oil when viewing through the 100X objective?: bc the light refracts the image to the viewer, the more power the objective lens has the easier you can view the image

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