lab 3
Terms
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- potential
- voltage difference b/w inside andoutside of neuronal membrane
- types of neuronal potentials
-
resting
synaptic
receptor
action - what causes potentials
- differnetial dist of ions on either side of neuronal membrane
- extracellular recording
- measure volt change along outisde of cell
- intracellular recoridng
- measures volt difference b/w inside and outside of cell membrane
- patch-clamp
- measure electrical current (ion flow) thru single ion channels in neuronal membrane
- optical imaging
- direct visualization of voltage diff cross cell membrane, provides sptail and temporal res of membrane potential
- steps of single AP
-
inhibitory hyperpolarization
sub-threshold depolarization
threshold depolarization
AP
after-hyperpolarization - differential amplifier
- determines and magnifies the difference b/w the two recording electrodes
- effect of changing polarity
-
SA: peak and trough flip
CAP: no effect - effect of changing amp
-
SA: amp increase as electrical stimulus increases
CAP: increase but there is a max - effect of changing freq
-
SA: increase # of SA
CAP: increase freq of CAP but there is max determined by abs. refractory period - effect of changing duration
-
SA: increase amt of time after electrical charge passes thru A before reaching B
CAP: no effect if stimulated at max amp; if not at max amp then amp may increase