Bio 333
Terms
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- Fastidious
- an organism that is difficult to grow in lab
- Brownian Movement
- Not moving with speed just shivering
- Lactose
- enteric pathogens intestinal, shigella and salmonella
- Mac Conkeys
- Gram Negative medium
- Medium
- No one medium that will grow everything, media will be steralized by exposure to steam at a pressure of 15 P.S.I.
- Advantage of MTA
- easily visible, live organisms all the time
- Monotrichous
- One flagellum at one end
- Media Requirements
- CHONSP
- Non-motile
- no movement
- peptone
- enzymatically hydrolized plant of animal tissue used in preperation of media
- Continuous Culture
- You add fresh nutrients and remove waste products so bacteria can continue to live in the culture
- Eosin Methylene Blue
- EMB, also used to isolate gram negatives
- Folmites
- Any innatimate object that can harbor microbes
- Disadvantage of MTA
- it takes a long time
- Most famous composition
- glucose minimal medium, only one carbon source
- Beta hemolysis
- the complete lysis of the red blood cells
- True Motility
- They will actually go somewhere has speed and direction
- Batch Culture
- finite (limited) amount of nutrients
- Growth Factors
- Amino acids, Purines and Pyramides, vitamins
- Gas Ethylenen Oxide
- Gas used to steralize Petri dishes and other heat labile material
- Can't grow in artifical medium
- Mycrobacterium Leprae, Chlamydia trachomatis, Treponema pallidum
- Mannitol Salt
- isolate staphylococcus species
- Peritrichous
- Flagella surround the cell
- Reducing Medium
- sodium thiogylcollate an example of reducing medium used to isolate and propagate anaerobes
- incineration
- making sterile, loopful turning red
- Atrichous
- No Flagella
- extract
- used in name typically shows media is complex
- MTA
- motility test agar
- Lac Positive and coliforms
- utilizes Lac+ as a substrate, a gram negative non spore forming rod that ferments lactose to acid and gas
- Selective and differential Media
- inhibit the growth of unwanted organisms
- Complex Media
- you do not know the exact chemical composition
- Selective Media
- antibiotics-vancomycin, bile salts-inhibit G+, Salt-Dehydrates and kills organisms, Dyes- MAL, CV, MB