Clin Lab VI Final
Terms
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- What conditions are ideal for a room used for microbiology testing?
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gas and electric outlets
fridge and incubators
washable floors
limited traffic
good lighting and ventilation
2 workspaces
ample storage space - What do you do when you are not sure of the correct stain to use?
- Read manufacturer's instruction
- Why do we innoculate milk sample
- check for mastitis
- Why do we innoculate intestinal infection
- enterobacteriaceae
- Why do we innoculate an eye specimen
- fastidious/found normally
- Why do we innoculate an ear
- malessezia (yeast) in dogs
- Diagnosis of cause of a disease is based upon what?
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clinical signs
gross pathologic observations
organisms involved
results of other lab tests - Synthetic media
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exclusively made from chemicals
exact chemical comp is known
experimental work
KOSER citrate broth - culture media
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any material, solid or liquid, that can support growth of microorganisms
most convenient - enriched media
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formulated to meet the requirements of the most fastidious pathogens
blood, serum and egg additives - selective media
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chosen antibacterial substances
brilliant green agar and MacConkey agar - differential media
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allow bacteria to be differentiated into groups by biochemical reactions
Simmons Citrate - basic nutrient media
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typically contain peptone, salt, dextrose, water,meat extract and solidifying agent
needed for growth - Blood agar
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enriched medium supports the growth of most bacterial pathogens, such as typticase soy agar
blood agar base
columbia agar
eugon agar - chocolate agar
- if Hemophilus infection is suspected
- McConkey Agar
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differential medium
crystal violet, which suppresses growth of gram+ bacteria - Mannitol Salt Agar
- not routinely used but a highly selective medium for staph
- Broths
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Tetrathionate broth is an enrichement broth for isolation of Salmonella
Selenite -used for isolation of Salmonella