17.1 quiz#1
Terms
undefined, object
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- nucleoli
- regions of RNA synthesis
- phase contrast microscope
- zernike, light travel faster or slower 1/4 wavelength than other deflected, creates degrees of brightness
- nucleus
- transcription
- SDS
- unfolds the tertiary structure, solubilizes proteins, adds negative charge
- fluorescent compound
-
DAPI
4'6 diamido 2 phenylindole - nonpolar amino acids
- have C's and H's, SH
- tertiary structure
-
3D conformation that results from folding of the polypeptide chain into a shape from hydrophobic and hydrophilic interactions
(ex: globular prots) - mercaptoethanol
- reducing agent(reduces disulfide bonds)
- all prots have
-
primary and secondary(alpha helix & beta plated sheets)
primary and secondary=1 polypeptide - electron beams
- small wavelengths, magnets used to focus the beams
- coiled coil
- amphipathic helix wrapping around another amphipathic helix, form dimers and polymerize, secondary structure
- ionic amino acids
- have an attached part with a - or positive charge
- hydrophobic aa residues within helix are spaced
- alternately 3 or 4 positions apart in the sequence
- higher percentage of agarose gel
- smaller pore size of gel making it better to separate small and shorter particles
- secondary antibody recognizes
- primary antibody coupled w/dye and is where fluorescence is coming from
- euk
- cytoskeleton-> basal bodies-> cilium
-
primary antibody
secondary antibody -
primary-IgG
secondary-antiIgG - immunofluorescence
- chemicals absorb light at 1 wavelength and emit at longer wavelength and less energy
- axoneme
- bundle of mts; bends due to sliding of mts, this bending moves the cilia
- electrophoresis
- based on a principle that charged molecules migrate in an electric field and separate charge and size
- denaturing electrophoresis
- prots are denatured w/sodium dodecyl sulfate and have a net negative charge
- indirect binding of secondary to primary antibody
- see specificities of primary antibody
- quaternary
- 2 or more indep folded prot chains loosely held together by weak bonds
- antibodies
-
prot molecules produced by B cells of vertebrates(immunoglobulins)
bind to foreign entities(antigens) w/high specificity(light and heavy chains)
- polar amino acids
- have OH groups, NH2 groups
- cytoskeleton
-
intermediate filamts
mts--dynein
actin--myosin - native electrophoresis
- separate proteins in a buffered environmt that maintains native charge and configuration
- primary antibody recognizes
- cell specific antigen