microbiology 1 osu
Terms
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- What is microbiology
- the study of microorganisms
- characteristics of microbes
-
-invisible to naked eye
-"fairly" simple organisms
- what is a unicellular organism?
- one cell makes up the entire organism
- what is a multicellular organism?
- more than one cell makes up the organism. Communication between cells (simple). Also usually in filaments and the cells are very similar.
- What are the eukaryotes?
- Fungi, algae, protozoa and slime molds/water molds
- What are teh prokaryotes?
- bacteria and archaea
- What are the acellular?
- viruses, prions, and viroids
- macroscopic
- visible to naked eye
- What did Robert Hook do?
- first observations of microbes, used microscope to describe tissue, described cells as the basic unit of organization of living things (must have cell to be alive)
- What are cells described as?
- basic unit of organization of living things
- Who is the father of microbiogy?
- Anton Van Leeuwenhoek
- Who described "animalcules"?
- Anton Van Leeuwenhoek
- What is spontaneous generation?
- living things just appear out of inanimate matter. People believed living things spontaneously appeared
- Who worked in teh French wine industry?
- Louis Pasteur
- Pasteur believed science should have _____________
- ...a practical application
- The use of heat to control microbial contamination is known as?
- Pasteurization
- An experiment where, microbes formed in meat broth in an open flask and didnt form in meat broth in a closed flask disproves what?
- spontaneous generation
- What is a swan neck flask?
-
flask with a thin, curved, straw-like opening
-open to the air and "life-force" however so thin that microbes get caught in the neck - What is the germ theory of disease?
- the idea that microbes are responsible for infectious disease
- Who studied anthraz and proved the germ theory of disease?
- Robert Koch
- When was the golden age of microbiology?
- 1857-1917
- When were foundations of microbiology and the importance of microbes established?
- During the Golden Age of Microbiology
- What is the age of molecular genetics?
- importance of DNA, beginning of genetic engineering, and more understanding of genes
- What did Hershey and Chase prove?
- DNA is a molecule of heredity, and they used microbes in their experiments
- Describe the Hershey/Chase experiment
-
-used bacteria and a virus
-labeled the DNA and the protein
-showed the DNA being transferred from one to the other - gene
- a segment of DNA molecule that provides the biochemical information for a function product
- What is metabolism?
- chemical reactions taking palce in a cell
- What is anabolism?
- making complex molecules from simpler ones
- What is catabolism?
- breaking down complex molecules into simpler ones
- Which requires energy, catabolism or anabolism?
- anabolism
- What is known as breaking down and building up?
- metabolism
- What is the universal energy currency?
- ATP
- organic compound
- a substance characterized by chains or rings or carbon that are linked to atoms of hydrogen and sometimes oxygen, nitrogen, and other elements
- photosynthesis
- anabolic, performed by phototrophs (must have light-absorbing pigments). getting energy from light
- How do non-phototrophs get their energy?
- glycolysis (the breakdown of glucose)
- Describe respiration
-
-requires air(performed by aerobes)
-during krebs cycle and ETS
-ends in 38 ATP, water and carbon dioxide
- Is respiration or fermentation more efficient?
-
respiration
- Define facultative anaerobes
- can use either respiration or fermentation
- Chemo=
- chemical, relies on breakdown of chemical compounds for energy, organic compounds
- photo=
- photosynthesis, relies on energy from light(sun)
- heterotroph=
- gets carbon from pre-formed organic compounds, other organism
- autotroph=
- gets carbon from carbon fixation, inorganic compound (carbon dioxide), must be converted into organic compound
- Chemoheterotroph
- chemical breakdown for energy, pre-formed organic compounds for carbon
- examples of chemoheterotroph
- e. coli
- photoheterotroph
- photosynthesis and pre-formed organic compound
- example of photoheterotroph
- rhodospirillum (purple sulfur bacteria)
- chemoautotroph
- chemical breakdown and carbon fixation
- example of chemoautotroph
- nitrobacter
- photoautotroph
- photosynthesis and carbon fixation
- example of photoautotroph
- cyanobacteria
- What is a carbohydrate
-
carbon(1)hydrogen(2)oxygen(1)
and its the energy source for cells - What is a monosaccharide?
- a simple carb, (one sugar), glucose, fructose, galactose
- What is a disaccharide?
- a two sugar carb. lactose, sucrose, maltose
- what is polysaccharide?
- many sugared carbohydrate. starch, cellulose
- What are 3 types of lipids?
- fats, phospholipids, and steriods
- What is a lipid
- contains carbon, hydrogen, and oxygen. insoluble in water, and a storage molecule
- What is a protein
- made up of 20 different amino acids, linked with peptide bonds
- What is an enzyme?
- a protein ending in -ase, that speeds up reactions in cells
- What are nucleic acids?
- stores genetic information in chemical code, composed of nucleotides(base and sugar)
- What are the 4(5) bases of nucleotides?
- A, T(U), C, and G
- What are 2 types of nucleotides?
- Ribonucleic Acid (RNA) and Deoxyribonucleic Acid (DNA)
- What are cell requirements?
- materials(organic molecules), driving force(ATP), and a plan(information stored in chemical code..DNA)
- In DNA A connects to _____
- T
- In RNA, A connects to ___
- U
- Why is DNA replicated?
- To copy DNA so a cell can divide, giving each cell a complete copy..
- How is DNA replicated?
-
-h bonds broken between bases (strands pulled apart)
-DNA polymerase, enzyme copies bases
-pool of nucleotides in cytoplasm
-phosphate backbone established - What is the purpose of DNA transcription?
- convert info in a form to direct metabolism, disposable copy of instructions
- What results in transcription?
- double stranded DNA is unchanged and single stranded RNA is made
- What are 3 types of RNA?
- mRNA, rRNA, and tRNA
- What is the purpose of mRNA
- to carry info needed to assemble amino acids into protein
- What is the purpose of rRNA
- to form the body of ribosome
- What is the purpose of tRNA
- to transfer or carry the appropriate amino acid to ribosome
- What is the purpose for DNA translation
- To produce protein from amino acids, the last step in gene expression
- What results in translation?
- A peptide chain is formed and is folded to make a protein
- What is a codon?
- 3 adjacent bases on mRNA, refers to the code for an amino acid
- Possible effect of a mutation can be?
-
-no impact at all
-slightly detrimental - Types of mutagens?
- UV radiation, environment or industrial chemicals, drugs, certain viruses
- What is taxonomy?
- the science of classification, uses established rules and classifies them by relatedness
- What are the 2 types of cells?
- prokaryotes and eukaryotes
- Are prokaryotes smaller or larger than eukaryotes?
- smaller, about 1/10th the size
- Which is simpler, prokaryotes or eukaryotes?
- prokaryotes
- "True Nucleus" refers to ____
- eukaryotes
- What are ribosomes?
- protein making structures, made of protein and RNA
- What does cell membrane (plasma membrane) do?
- barrier between the inside of the cell and environment, regulates what enters and leaves the cell, communicates with external environment
- What is cell membrane composed of?
- phospholipids
- What is the cytoplasm?
- the matrix of the cell, its a gel-like liquid, provides a fluid medium for the cell
- What is a genome?
- chemical form of the information storage, DNA/RNA
- Single, circular chromosomes refers to what?
- prokaryotes
- several, linear chromosomes refer to ?
- eukaryotes
- Where are organelles found?
- eukaryotes only
- What is generated in the Mitochondria?
- ATP (energy), only found in eukaryotes
- Do both prokaryotes and eukaryotes have cell walls and flagella?
- no, they may or may not have cell walls and flagella
- What are flagella?
- whiplike structures (generally give the ability to be mobile)
- How do eukaryotes reproduce?
- asexual and sexual
- How do prokaryotes reproduce?
- asexual
- What is a group of similar species?
- genus
- What are organisms that can be interbred and have a common pool of genes?
- species
- Species that differ by 1 characteristic are called a ?
- strain
- What is a virus composed of?
- protein and nucleic acid
- Does a virus need a host to reproduce?
- yes
- Define virus:
- any unseen or unknown entity involved in infectious disease
- What is helical structure?
- tightly wound coil
- What is a icosahedral structure?
- geometrical figure with 20 equal-sized triangular faces
- what is a complex structure or a virus?
- combination structure and/or has additional structures on it
- what is the nucleocapsid
- genome + capsid
- How do you cultivate viruses in bacteria?
- bacteriophage..grow bacteria in a broth and add bacteriophage
- What is a bacteriophage?
- "bacteria eater" virus that infects bacteria
- How do you cultivate viruses in plants?
- By infect the whole plant
- How do you cultivated viruses in animals?
- infecting host animals, infecting fertilized chicken eggs, or with a cell culture/cell line
- What are 2 properties of cancerous cells?
- grow and divide more frequently, stick together less firmly, undergo dedifferentiation(going back to earlier stage of development..looking like stem cells)
- What are proto-oncogenes?
- normal genes that regulate growth, cant be converted to oncogenes by viruses
- oncogenes
- genes that can transform cells into cancerous cells
- What is a viroid?
- circular molecule of single stranded RNA, no capsid, infects plants only
- Potato Spindle tuber disease is an example of what type of infectious agent?
- viroid
- What is a prion?
- composed of protein only, infects central nervous system of humans and animals, altered form of normal host proteins corrupts protein
- Mad Cow Disease is caused by what?
- a prion
- What is the cell membrane linkage for archaea?
- ether
- What is the cell membrane linkage for bacteria?
- ester
- What have cells walls of pseudomerein?
- archaea
- What make up the cell walls of bacteria?
- peptidoglycan
- What nutritional classes are archaea?
- chemoautotroph or chemoheterotroph
- What nutritional class are most bacteria?
- chemoheterotroph
- What are 3 traits of all prokaryotes?
- simple cells, no organelles, and asexual reproduction
- How does binary fission work?
- one celle splits into 2 identical copies
- What color is gram+ stains?
- purple or blue
- what color are gram- stains?
- red or pink
- DOes gram+ or gram- bacteria contain many, many layers of peptidoglycan?
- gram+
- Does gram+ contain teihoic acid?
- yes
- What shape is bacilli?
- rod shaped, can form long chains
- What shape is cocci?
- round, circular
- What shape is spiral?
- curved rods
- Describe the glycocalyx
- outermost layer of cells, either a capsule or slime layer, and provides protection allowing bacteria to cause disease
- What are the pili used for?
- transferring DNA from one cell to another, and attachment to tissue or other surfaces
- Antibiotic resistance if often transferred through what?
- plasmid
- What is the most resistant biological structure known?
- endospore
- What is "animal-like"
- fungi
- What nutritional class are fungi?
- chemoheteroph
- Where do saprobes get organic material from?
- dead/decaying organisms
- What are examples of fungi?
- yeasts, molds, mushrooms
- How does yeast reproduce?
- budding
- What fungi is associated with animal feces?
- zygomycota
- What fungi are mushrooms?
- basidiomycota
- What fungi(yeast) are used in the production of cheese, bread, and beer?
- ascomycota
- Define mutualistic:
- beneficial to both organisms
- Are lichens single organisms?
- no
- About 10% of a lichen is made up of what?
- a photosynthetic microbe