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Terms
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- Phenylalanine
-
POS: Proteus, mirabilis, providencia
neg: everything else - Test after phenylalanine; what it shows:
-
Indole: shows
The only neg is -> P. mirabilis
Pos -> Do an ornithine - Ornithine results of phenyl/indole POS
-
Ornithine pos: Morganella
Ornithine neg: do an H2S to differentiate P. vulgaris and Providen. - differentiation of P. vulgaris and Providencia:
-
H2S:
pos = p. vulgaris
neg = providencia - What test is done on Phe Neg bugs
- Voges Proskauer
- VP results:
-
POS = klebsielliae tribe
Klebsiella, Enterobacter, Serratia
Neg = SEE the SCY
Salmonella, E. coli, Edwardsiella, Shigella, Citrobacter, Y. enterocolitica -
1st test to run on VP pos organisms
Results? -
Indole;
Pos: Klebsiella oxytoca
Neg: all else; do a Lys/Ornithine - On VP pos/Indole neg bugs, what test to run, what is the result?
-
Lysine/Ornithine.
Lys+/Orni- -> Klebsiella pneumoniae.
Any other combo -> others -> do DNAse - What test is done on VP pos after lys/ornithine on "all else"?
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DNAse or Gelatin
DNAse pos = Serratia
Dnase neg = Enterobacter - What organisms are Phe neg, VP neg?
- See the Scy
- First test to run on Phe neg, VP neg:
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Lysine
Pos = SEE;
Salmonella, Edwardsiella, E. coli
Neg = SCY
Shigella, Citrobacter, Yersinia - 1st test on Phe +, VP -, Lys pos:
-
H2S;
Only neg = E. coli
Pos = do an INdole - Indole result of Phe pos, VP neg, LYS pos, H2S pos:
-
Differentiates Edwardsiella/Salmonella:
Indole
Pos = Edwardsiella
Neg = Salmonella - First test to run on Phe neg, VP neg, LYS neg:
-
H2S:
Pos: C. freundii
Neg: do a Citrate - what test is run on Phe neg, VP neg, Lys neg, H2S neg?
-
Citrate;
Pos = Citrobacter koseri
Neg = Yersinia/Shigella - do a TSI - Last test to run on Phe neg, VP neg, Lys neg, H2S neg, Citrate neg:
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TSI:
Pos = Yersinia enterocolitica
Neg = Shigella
YES! - If it's citrate positive, isolate cannot be:
-
SEE MY
Shigella, Edwardsiella, E. coli
Morganella, Yersinia enterocolitica - If it's H2S positive, isolate can only be
-
SPEC
Salmonella
Proteus
Edwardsiella
Citrobacter freundii - If Urea is 4+ isolate can only be:
- Proteus, Morganella, some Providencia
- If TSI is A/A(g) isolate cannot be:
-
shigella, edwardsiella, Proteus mirabilis, Morganella, Providencia
These are always K/A only glucose!!! - the isolates that are NEVER A/A are:
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PEMPS
Edwardsiella, Morganella, P. mirabilis,
Providencia, Shigella - If lysine is deaminated, the isolate can only be:
- Proteus, morganella, or providencia
- If VP is positive, isolate can only be
-
Klebsiella oxytoca/pneumonia
Enterobacter
Serratia
50% of Proteus mirabilis - If motility is pos at 37 degrees C, isolate cannot be:
- Shigella, Klebsiella, or Yersinia
- Of the klebsiellas, which is indole pos/neg?
-
K. oxytoca: Indole pos
K. pneumoniae: neg -
Of the enterobacters, which are ornithine pos?
neg? -
E. cloacae
E. aerogenes
Neg = E. agglomerans - How to tell Enterobacter agglomerans from other species:
- Neg on Ornithine, lysine, and arginine
- How to tell Enterobacter cloacae from aerogenes and agglomerans:
- +ornithine, +arg, -neg lysine
- How to tell Enterobacter aerogenes from Enterobacter cloacae and Enterobacter agglomerans:
- +Pos ornithine, -Neg arginine, Pos lys
- How to speciate Enterobacter:
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Orn Arg Lys
Cloacae + + -
Aerogenes + - +
Agglomerans - - - - How to speciate Citrobacter:
-
H2S Indole
C. freundii + -
C. koseri - + - How to speciate Proteus:
-
Indole Ornithine
Mirabilis - +
Vulgaris + -