Microbiology Lab
Terms
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- What do Gram Positive cocci produce?
- Endotoxins called hemolysins
- Hemolysins are capable of what?
- Destroying red blood cells and hemoglobins
- What are three major types of Hemolysins?
- Beta, Alpha and Y
- What do Beta Hemolysins do?
- Complete destruction of RBCs and hemoglobin. It results in a clearing of the medium around the colonies
- What do Alpha Hemolysins do?
- Partial destruction of RBCs and produces a greenish discoloration of the AGAR around the colonies
- What do Y- Hemolysins do?
- It is non hemolysis and appears as a simple growth with no change in the medium
- The hemolysins produced by streptococci are called what?
- Streptolysins
- What are the two forms of streptolysins?
- Streptolysin O and Streptolysin S
- What is blood Agar especially useful for?
- Detecting Hemolytic ability of Gram Positive Cocci and as a general purpse growth medium appropriate for fastidious and nonfastidious microogranisms alike
- Describe the Streptolysin O
- IT is oxygen-labile and expresses maximal activity under anaerobic conditions
- Describe the Streptolysin S
- It is oxygen-stable but expresses itself optimally under anaerobic conditions as well
- How do you innoculate blood agar to make it favorable for streptolysins?
- Streak-stab technique
- How do the stabs encourage streptolysin activity?
- because of the reduced oxygen concentraion of the subsurface environment
- Why is snyder test medium formulated?
- To favor the growth of oral bacteria and discourage the growth of other bacteria
- What is Epidemiology?
- the study of the causes, occurence, transmission, distribution, and prevention of diseases in a population
- The snyder test is designed to meaure susceptibility to what?
- Dental Caries
- What is Dental Caries primarily caused by?
- Lactobacilli and streptococci
- Name three ways infectious diseases are transmitted
- inhalation, direct skin contact, blood to blood contact
- What is Common Source epdiemic?
- When a disease is transmitted from an area such as a heating or cooling system of a building or contaminated water it infects many people all at once.
- What is propagated transmission
- a disease transmitted from person to person
- The first case of such a disease is called te ____ ____
- Index Case
- How do you calcuate incidence rate
- Number of new cases in a time period over size of at risk population at midpoint of time period X K
- How is point Prevalence calculated?
- Number of existing cases at a point in time over the total population X K
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