Microbial Metabolism
Terms
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- What changes the shape of the active site of an enzyme
- Non competitive inhibitor
- What is very similar in shape or chemistry to the normal enzyme substrate
- Competitive Inhibitor
- What is a photosynthetic bacteria that uses carbon dioxide as a carbon source
- Photoautotroph
- Both carbon and energy usually with the same organic compound
- Chemoheterotroph
- What is photosynthetic and uses organic material as a carbon source
- Photoheterotroph
- What is a mechanism which fatty acids are degraded
- Beta Oxidation
- A group of enzymes that function as electron carriers in respiration and photosynthesis
- Cytochromes
- A protein portion of an enzyme, inactive without a co factor
- Apoenzyme
- A measure of the rate of activity of an enzyme
- Turnover Number
- A non protein component of an active enzyme
- Coenzyme
- What is a "Whole enzyme"
- Holoenzyme
-
Building of complex molecules. Requires energy
ATP Breakdown - Anabolism
- Breakdown of organic compounds. Releases energy. ATP synthesis
- Catabolism
- What are sequences of chemical reactions
- Metabolic Pathways
- Reduce activation energy of a reaction~Has a three dimensional shape~Interact with a substrate molecule~serve as a catalyst
- Enzymes
- Enzymes are important in living organisms because they......
- bring together reactants or properly orient a molecule for a reaction
- What assists an enzyme by accepting or donating matter
- coenzyme
- What is NAD
- Electrons
- In bacteriologic media trace elements are required because...
- They serve as sources of enzyme cofactors
- What does competitive inhibition of enzyme action involve?
- Completion with the substrate for binding at the active site
- What is ATP?
- Adenosine triphosphate. Stroes the energy rec'd from catabolic reactions and releases it later to drive anabolic reactions.
- The reaction that is coupled with the oxidation of a substrate...
- NAD
- Oxidation of glucose involves what 3 stages?
-
1. Glycolysis
2. Krebs Cycle
3. Electron transport chain - During glycolysis electrons from the oxidation of glucose are transferred to
- NAD
- NADH formed during glycolysis and in Krebs cycle are...
- oxidized when electrons are passed to the electorn transport chain
- ATP produced during Krebs cycle is formed by
- Substrate level Phosphorylation
- In Aerobic electron transport chain what happens last?
- Electrons are transferred to Oxegyn
- The Proton Motive force is..
- An electrochemical gradient formed across a membrane
- Unlike Eukaryotes, in prokaryotes chemiosmosis
- Occurs at the plasma membrane and not the mitochondria.
- What controlls all Enzymes (Protein)
- DNA
- Shapes change when heated because
- It is a protein
- What is Penose-Phosphate pathway
- Heterotrophs use organic molecules as energy and carbon sources needed for nucleic acids.
- What is Anaerobic resperation
- Bacteria use oxygen substitutes such as nitrates
- Pyuvic acid accepts electons and is turned into various end products
- Fermentation
- Glucose to pyruvic acid
- Glycolysis
- Electons are removed from an organic compound and transferred by an electon transport chain to oxygen
- Oxidative phosphrylation
- An electron is liberated from chorophyll and passes down an electron transport chain
- Photophosphorylation
- A dehydrogenase coenzyme derived from nicotinic acid. Functions as electron carrier. Is Catabolic
- NAD
- A dehydrogenase coenzyme derived from riboflavin
- ATP synthase
- In chemiosmosis, protons can diffuse across a membrane only through special channels that contain this enzyme
- FMN
- Pyruvic acid loses carbon dioxide to form an acetyl group
- Decarboxylation
- Glycosis
- Embden-Meyerhof
- A photosynthetic organism that does not produce oxygen
- Anoxygenic
- Removal of electrons
- Reduction
- Uses an inorganic source of energy such as ammonia or elemental sulfur
- Chemoautotrophic
- A chemoheterotroph that lives on dead organic matter is called
- Saprophyte
- When an enzyme's active site is occupied at all times by substrate or product molecules it is
- Saturated
- Cyanide is an example of a general type of inhibitor called
- non competative
- Sulfa drugs are an example of a type of inhibitor called
- Cometator
- In substrate level phosphorylation
- no oxygen or other inorganic final electron acceptor is required
- Deamination is
- the removal of NH2 from an amnio acid
- Decarboxylation is
- thr removal of COOH from an amino acid
- Substrate is
- The substance acted upon by an enzyme
- Glucos is usually broken down to pyruvic acid by
- Glycolysis
- In aerobic respiration, pyruvic acid is converted to acetyl COA then can
- enter Krebs cycle
- DNA and RNA are made up of repeating units called
- Neucleotides
- When phosphate is split from ATP
- ADP is formed
- Tertiary
- Most enzymes are in this shape
- Where does protein synthesis happen?
- In the kitchen (Ribosomes)
- One enzyme for each
- Chemical reaction
- Co Factor
- Minerals
- Co Enzyme
- Vitamins
- NAD
- Vit B3
- FAD
- Vit B2
- Cellular Respiration
- Inorganic
- Fermentation
- Does not requier Krebs cycle or an electron chain. Organic
- Competitive ingibitors
- Bind to enzyme active site. Where the puzzle would fit.
- Non competitive inhibitors
- bind to an allosteric site
- Oxidation
-
removal of electorns
When a molecule is oxidized it loses energy - Reuction
-
gain of electrons
When a molecule is reduced it gains energy - Redox
- When one molecule loses elecrons, another gains them. It is when reduction reactions are paired with oxidation reactions.
- Apoenzyme
- Enzyme with a portion of a protein
- Cofactor or coenzyme
- Non protein component
- Coenzyme
- Intermediat energy carrier from one pathway to another.
- Substrate level phosphorylation
- direct transfer of phosphate from substrate to ADP to make ATP
- Oxidative phosphorylation
- involves electron transport chain
- Chemiosmosis
- production of ATP from DP through electrochemical gradient across a membrane.From a buildup of protons
- Photophosphrylation
- Converts solar energy into chemical energy
- Catabolism
- Most micros use glucose or carbs as energy. Lipids and protiens also used.
- Cellular resperiation
- ATP process food molecules oxidized. Electron transport carriers.
- Aerobic respiration
- Electron acceptor is O2.
- Anaerobic respiration
- Acceptor is a inorganic molecule. Only happens in bacteria
- Glycolysis
- splitting of sugar
- Metabolic pathways
- sequences of chemical reactions determined by its enzymes that are determined by cells genetic makeup
- NADP
- Electron carrier. Is anabolic. Requires energy
- Co enzyme A
- Diriv of B Vit important in synthesis and breakdown of fats in Krebs cycle
- Some cofactors are metal ions that may help
- Catalyze enzymes, bridging.
- Competitive enzyme inhibitor
- compete with normal substrate for active size on enzyme
- Noncompetitive enzyme inhibitor
- decrease ability of normal subsrate to combine with enzyme. Inhibitor changes shape of active site making it nonfunctional.