MMG Lab 11
Terms
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- What are some physical ways to control growth of microbes?
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-Heat
-Pasteurization
-ultra-high-temperature
-autoclaving
-UV light - What are some chemical ways of controling microbial growth?
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-dyes
-antimicrobial agents (such as antibiotics) - What are the settings for autoclaving?
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-121 C
-15 minutes
-15 pounds pressure - How does UV light work to sterilize things?
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-light absorbed by purine and pyrimidine bases of DNA, cuasing thymine dimers
-orgnaism can't replicate and dies - What are thymine dimers?
- -the binding of two adjacent thymine residues
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What types of organisms are most susceptible to UV light?
Most resistant? -
-actively dividing cells
-spores and pigmented cells - What is difficult about UV light?
- -Poor penetration ability
- What is a common dye used in chemical control of microbes?
- -Crystal Violet combats thrush
- What are the two categories of antimicrobials?
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-bacteriostatic
-bacteriocidal - What do antimicrobials work on in the microbe?
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-cell wall synthesis
-ribosomal action
-nucleic acid synthesis
*may act on some other cellular site - When treating with antibiotics, what must be considered?
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-degree of susceptibility
-toxicity to host - What are 5 ways to test for microbe sensitivity to antimicrobials?
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1) classic tube dilution
2) micro-dilution method
3) agar plate dilution
4) Kirby-Bauer disc diffusion methods
5) E-test (new)