micro/talaro
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- What are the 5 I's of studying microorganisms?
-
1.inoculation
2.incubation
3.isolation
4.inspection
5.identification - List 4 examples of different types of microbiological media.
-
broth
enriched
agar
gel - What is the term that refers to the purposeful addition of microorganisms into a laboratory nutrient medium?
- inoculation.
- Which is essential for development of discrete, isolated colonies?
- solid medium
- A pure culture contains only...
- one species of microorganism
- What type of medium will result when 1% to 5% agar is added to nutrient broth, boiled and cooled?
- a solid medium
- Agar is an important component of media because...
- agar provides a solid surface for bacterial growth
- What is a contaminated culture?
- When one organism growing in a medium is somehow contaminated with another.
- Bacterioides is inoculated into a pour plate. After incubation, colonies are only seen within the agar and not on the surface. Bacterioides must be...
- anaerobic.
- A microbiologist inoculates Staph. epidermidis and E. coli into a culture medium. Following incubation, only the E. coli grows in the culture. what is the explanation?
- The culture medium must be selective.
- Which method often results in colonies developing down throughout the agar and some colonies on the surface?
- pour plate.
- A common medium used for growing fastidious bacteria is...
- Blood agar.
- A nutrient medium that has all of its chemical components identified and their precise concentrations known and reproducible, would be termed...
- synthetic.
- A reducing medium contains...
- substances that remove oxygen.
- Which type of medium is able to distinguish different species or types of microorganisms based on an observable change in the colonies or in the medium?
- differential
- When one uses a nutrient medium that contains thioglycollic acid, what type of microbe is one attempting to culture?
- anaerobe
- List 4 growth characteristics that occur using differential media?
-
1.different color colonies
2.different media color post incubation
3.precipitates
4.gas bubbles - A reducing media is used to culture...
- anaerobic organisms
- For which bacterial genus is mannitol salt agar selective?
- Staphylococcus.
- In culturing a patients feces for intestinal pathogens, what is likely to be present in selective media for analyzing this fecal specimen?
- bile salts
- What is a typical characteristic that refers to the microscopes ability to show two separate entities as separate and distinct?
- resolving power
- What part of the microscope magnifies the specimen to produce the real image of the specimen?
- objective lens
- What magnifies the specimen to produce the virtual image of the specimen?
- ocular lens
- If a microbiologist is studying a specimen at a total magnification of 950X, what is the magnifying power of the objective lens if the ocular lens is 10X?
- 95X
- What is the limit of cell diameter that would be resolved in a microscope/
- 0.2um
- The wavelength of light used plus the numerical aperture governs...
- resolution
- The type of microscope in which you would see brightly illuminated specimens against a black background is...
- dark-field
- This microscope does not employ light in forming the specimen image:
- electron. It also achieves the greatest resolution and highest magnification.
- This scope shows cells agaist a bright background and also shows intracellular structures of unstained cells based on their varying densities:
- phase-contrast
- This microscope cannot image live specimens:
- scanning electron
- This scope is the most widely used and shows cells against a bright background:
- bright-field
- List 4 characteristics that pertain to the flourescence microscope?
-
1.type of compound microscope
2.requires the use of dyes like acridine and fluorescein
3.commonly used to diagnose certain infections
4.requires an ultraviolet radiation source - Which microscope bombards a whole metal coated specimen with electrons moving back and forth over it?
- scanning electron
- The specimen preparation that that is best for viewing cell motility is..
- hanging drop
- The Gram stain, acid fast stain and endospore stain have the following in common:
- outcome based on cell wall differences
- Basic dyes are...
- attracted to the acidic substances of bacterial cells
- A biologist makes a fixed smear of bacterial cells and stains them with Loefflers methylene blue. All the cells appear blue under the oil lens. This is an example of:
- simple staining
- The 3 physical forms of laboratory media are:
- solid/semisolid/liquid
- Bacteria that require special growth factors and complex nutrients are termed...
- fastidious
- This is a term used for a culture made from one isolated colony...
- both axenic and pure
- Newly inoculated cultures must be_______ at a specific temperture and time to encourage growth.
- incubated
- The _____ of the microscope holds and allows selection of the objective lenses.
- stage
- ______ dyes have positive charges on the chromophore and are repelled by bacterial cells.
- acidic
- ______ has the same optical qualities as glass and thus prevents refractive loss of light as it passes from the slide to the objective lens.
- immersion oil
- The procedures for culturing a microorganism requires the use of a microscope. TRUE/FALSE
- FALSE
- One colony typically develops from the growth of several parent bacterial cells. TRUE/FALSE
- FALSE
- Some microbes are not capable of growing on artificial media. TRUE/FALSE
- TRUE
- Mixed cultures are also contyaminated cultures. TRUE/FALSE
- FALSE
- A medium that is gel-like has less agar in it compared to a solid medium. TRUE/FALSE
- TRUE
- A selective medium contains one or more substances that inhibit growth of certain microbes in order to facilitate the growth of other microbes. TRUE/FALSE
- TRUE
- A bacterial species that grows on blood agar but will not grow on trypticase soy agar is termed an anaerobe. TRUE/FALSE
- FALSE
- Fixed smears of specimens are required in order to perform the Gram stain and endospore stain on the specimens. TRUE/FALSE
- TRUE
- The bending of light rays as they pass from one medium to another is called refraction. TRUE/FALSE
- TRUE
- At the end of the Gram stain, Gram positive bacteria will be seen as pink cells. TRUE/FALSE
- FALSE
- Scanning tunneling microscopes can give close up views of DNA molecules, while the scanning probe microscopes have the potential to see individual atoms. TRUE/FALSE
- FALSE