Research Methods 1 final
Terms
undefined, object
copy deck
- How many times does IACUC meet?
- once a month
- what does IACUC stand for?
- Institutional Animal Care and Use Committee
- What are four of the five members of IACUC
- Vet, Scientist, Lay member, Unaffiliated person, Clergy/ethicist/lawyer
- what are the 8 ethical groups?
- Religious, Utilitarian, Natural law, Naturalism, Rights, Alturism, Deontology, Prima Facie, Universalist
- what are the six ethical rights?
- negative, positive, natural, legal, human, moral
- what are the seven prima facie foundations?
- Promise keeping, reparation for harm done, gratitude, justice, beneficence, self-improvement, non-malfeasance
- what is the definition of euthansia?
- a humane way of ending an animals life without causing pain or distress
- what is an important aspect for a euthanasia agent?
- renders the animal unconcious before dying
- define Congenic
- an inbred strain that differs from arent inbred strain at one gene
- define Isogenic
- an inbred strain that does not differ from parent inbred strain
- define F1 hybrid
- genetically uniform population of animals with numerous heterozygous gene produced mating two different inbred strains.
- Define SPF
- Specific Pathogen Free- tested for specific pathogens, does not mean disease free
- define VAF
- Varified Antigen Free- free of known diseases
- Define Axenic
- germ free
- what are the cage height requierments for mouse, rats, hamsters, gerbails G pigs and rabbits?
-
mouse - 5
R/G/H- 7
GPig-8
Rabbit-14 - Genus and species name for cat?
- Felis catus
- Genus and species name for dog?
- Canis familiaris
- Genus and species name for Ferrets?
- Mustela putorius furo
- Genus and species name for chinchilla
- Chinchilla laniger
- Genus and species name for Gerbils
- Meriones unguiculatus
- Genus and species name for rabbit?
- Oryctolagus cuniculus
- Genus and species name for Xenopus
- Xenopus sp.
- Genus and species name for zebra fish
- Danio rerio
- Genus and species name for hamsters
- Mesocricetus auratus
- Genus and species name for Guinea pigs?
- Cavia porcellus
- Genus and species name for Mouse
- Mus musculus
- Genus and species name for rats
- Rattus norvegicus
- what does a nude mouse lack?
- thymus
- what are the five analgesic drug classes and example of each?
-
opioid agonist - morphine
opioid agonist/antagonist- butorphanol
dissociative- ketamine
NSAID- asprin
Barbituates- pentobarbital
Benzodiazepine- diazepam - what is the genus and secies of a Rhesus monkey?
- Macaca mulatta
- what are the four types of transgenic mice?
- stud, vasectomized male, donor mouse, recipient mouse
- unique features of hamster? ferret? chinchilla? gerbils?
-
H-cheek pouches, hybernate when cold
F- can bend spine in half
C-dust bath, fur slip
G- seize when loud noise - how much space does a 4 kg rabbit need?
- 4 ft squared
- how much space does a 26 g mouse need?
- 15 square inches
- how much space does a 250 g rat need?
- 29 square inches
- how much space does a 400 g rat need?
- 40 square inches
- what are two diseases caused by bacteria that zebra fish get?
-
Velvet disease- caused by Oodinium pillularis
Mycobacteriosis- fish TB caused by Mycobacterium marinum - how should you prevent diseases in zebra fish?
- washing hands, not letting water mix, sterilizing all equipment, cleaning water, reduce stress, eliminate sick fish
- water caused troubles to zebra fish?
- copper tozicity, ammonia tox, gas bubble disease
- what does allodynia mean?
- when nerve endings become exhausted an ordinary stimulus previously innocuous, may generate a noxious reaction
- define nociception
- nerve pathway that transmits pain stimulus
- define afferent pathway
- pathway connecting afferent nerves connecting PNS to CNS
- what are methods of assesing pain?
- posturing, vocalizing, anorexia, guarding, inability to right, loss of homeostasis, rapid breathing
- signs of distress?
- vocalization, anorexia, diarrhea, hunched posture, ruffled fur, porphyrin tears
- assesment of pain?
- interacting w/cage mates, crying, limb withdrawal, biting, redness, temp, loss of function, resume normal behavior quickly
- difficulties in assesing pain?
- lack of knowledge, lack of awareness of protocol by staff, lack of access to class drugs, great variability among species in pharmacodynamics, lack of objective criteria to asses pain
- in the animal welfare act what are the three areas of respect to sanitation?
-
- removal of excreta
- minimizing spread of disease
- maintaining odors at low level - difference between latex gloves and nitrile gloves?
-
nitrile blue, are puncture resistant, 100% tested
latex not largly tested, some high blowout rate