MMG Applied Microbiology
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- What are some applications where industrial microbiology is used?
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-industrial products
-food microbiology (fermentations)
-treatment of water and wastewaters
-disposal and cleanup of biological wastes
-treatment of mine drainage - What is Industrial production?
- -large-scale growth of particular microbes for producing some type of beneficial compound such as AA or vit
- What are primary metabolites?
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-produced during active growth and metabolism
-either required for the production of new organisms or are by-products of active metabolism - What are secondary metabolites?
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-produced after cells have left log phase of growth and have entered the stationary phase
-substabces are not immediately needed for growth - In industrial microbiology, what are products prosduced as?
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-primary metabolites by the organism
-secondary metabolites by the organism - What do recombinant organisms add to the chemicals produced by bacteria during their metabolic activities?
- -add diversity by producing substances not normally manufactured by microbial cells
- What are some advantages offered by microbial processes?
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-use renewable resources
-environmentally benign
-synthesis of compounds too complex for exonomic chemical synthesis (ie: chiral synthesis, enantiomerically-pure chemicals) - What are some enzymes produced by microorganisms and their uses?
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-amylase and proteases: spot removers
-streptokinase: breakdown of blood clots
-restriction enzymes, ligases, polymerases: molecular biology/reasearch - What are some food additives/supplements produced by microorganisms and their uses?
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-amino acids, vitamins: health supplements
-citric acid: antioxidant
-sorbic acid: food preservative - What are some industrial products produced by microoorganims and their uses?
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-Indigo: dye used in manufacturing clothes
-Plastics: biodegradable substances for petroleium-based plastics - What are some alternative fuels produced by microorganisms and their uses?
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-ethanol: used in gasoline
-methane: burned to generate heat and electricity
-Hydrogen, hydrocarbons: potential fuels -
What are some pharmaceuticals produced by microorganisms and their uses?
+Pesticide? -
-antimicrobial drugs: tx of bacterial infections
-insulin, human growth hormone: replacement hormones
-taxol: cancer treatment
+Bt toxin: insecticide - What makes Vitamin B12?
- -Propionibacterium, Pseudomonas strains
- What makes Vitamin B2?
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AKA riboflavin
-made by Ashbya gossypii at 7g/L - What are 6 properties of a useful industrial microorganism?
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-makes a valuable product
-grows and performs well in large-scale cultures
-fast growth and production
-grows in inexpensive media (corn steep liquor, whey)
-non pathogenic
-amenable to genetic manipulation, genetically stable - What are some attributes food receives from fermentation?
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-flavors
-aromas
-consistencies - What is fermentation in food microbiology?
- -any desirable changes that occur to a food or beverage as result of microbial growth
- Which products are best known for natural microbial populations capable of fermentative metabolism?
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-grains
-fruits
-vegetables - What is a starter culture in food microbiology?
- -composed of known microorganisms that perform specific fermentations consistently
- What are some fermented vegetables and what do they make?
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-cabbage: sauerkraut/kimchi
-cucumbers, peppers, beets: pickles
-soybeans and wheat: soy sauce
-Rice and soybeans: Miso - What is alcoholic fermentation?
- -various species (predominantly yeasts) convert simple sugars into alcohol (ethanol) and CO2
- What are some intrinsic factors that can cause food spoilage?
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-nutritional composition
-water activity
-pH
-physical structure
-microbial competition - What are some extrinsic factors that can affect food spoilage?
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-degree of processing
-amount of preservatives
-storage temperature
-storage packaging - What are perishable foods?
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-often nutrient rich
-moist
-unprotected by rinds or coverings
-kept cold - What are semi-perishables?
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-can be stored in sealed containers for months without spoiling if unopened
-many fermented foods are semi-perishable - What are non-parishable foods?
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-dry or canned foods that can be stored alomst indefinitely w/o spoiling
-often are nutrient poor, dried, fermented, preserved - What are 6 ways of preventing food spoilage?
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-industrial canning
-pasteurization
-lyophilization (freeze drying)
-gamma radiation
-preservatives
-high and low temps - What are some different types of preservatives?
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-salt and sugar remove water from food
-garlic contains allicin which inhibits enzyme function
-benzoic acid interferes with enzymatic function
-certain spices produce oils that interfere with membranes
-chemical preservatives may inhibit microbial growth but don't kill microbes - What does industrial canning do to prevent food spoilage?
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-heating eliminats meophilic bacteria and endospores
-hyperthermophilic microbes remain but can't grow at room temperature - What does pasteurization do for food spoilage?
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-lowers number of microbes but some microbes survive
-used most with delicate foods and beverages - What does freeze drying do to preven food spoilage?
- -foods are frozen and then a vacuum draws off the ice crystals
- What does gamma radiation do to prevent food spoilage?
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-can penetrate food and damage the DNA of microbes
-can achieve complete sterilization - What two groups can food-poisoning be broken into?
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1) food infections
2) food intoxications - What is food infection?
- -result from the consumption of living microorganisms
- What is food intoxications?
- -caused by consumption of microbial toxins rather than the microbe itself
- What are the steps in water treatment?
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-sedimentation and flocculation to remove large materials
-filtration using sand, activated charcoal, or membrane filtration to remove microbes
-disinfection using chlorine, ozone, or UV light to inactivate remaining microbes - How is the majority of water-borne illnesses spread and caused by?
- -fecally contaminated water
- How is water quality testing often done?
- -uses the presence of certain indicator organisms to indicate the possible presence of pathogens in drinking water or natural aquatic systems
- What to bacterium often indicate other pathogens may be present?
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-E coli
-fecal coliforms - Why is E. coli a good indicator organism?
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-consistently found in human waste
-survives in water as long as most pathogens
-easily detected by simple tests such as membrane filtration, ONPG, and MUG tests - What is membrane filtration?
- -plating of a sample and counting colonies
- What is the purpose of wastewater treatement?
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-to remove or reduce contaminants and microorganisms to acceptable levels
-reduce the biochemical oxygen demand (BOD) - What is BOD?
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-biological oxygen demand
-a measure of the amount of oxygen that aerobic bacteria require to metabolize wastes in water - What are the three components to domestic wastewater?
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-organics
-inorganics
-microbial - What are the requirements for effluent to be released to environment?
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-BOD < or equal to 20mg/L
-N < or equal to 18mg/L
-E coli <equal to 10mg/L - What are three different types of municipal wastewater treatment facilities?
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-physical
-biological
-chemical - What are nonmunicipal wastewater treatment?
- -septic tanks and cesspools
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What happens to sludge in municipal treatments?
How about nonmunicipal -
-anaeorobically digested and then:
-dried for lanfill or ag
-and methane burned off or trapped for fuel
+sludge stays in tank and must be eventually pumped out - In a complex exosystem, what bacteria is most interactive with activated sludge?
- -Gr + Actinomycetes