5. BUN and Creat methodologies
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- List the 2 main types of BUN methodologies:
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1. Indirect methods
2. Direct methods - List the 3 indirect BUN methods?
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1. Coupled enzymatic
2. Bertholot
3. Conductimetric - What is the direct BUN method?
- Diacetyl monoxime
- What is the basic principle of the indirect BUN methods?
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-Urea is converted to CO2 NH4 ion;
-The ammonium ion is then measured. -
What enzyme is used for "Coupled Enzymatic BUN method"?
What is ultimately measured? -
-Urease enzyme
-Final measurement: NAD - What wavelength is NAD measured at?
- 340 nm
- What is ultimately measured in the Berthelot method?
- Indophenol Blue product
- Why is the Berthelot method less dependible?
- Ammonia in the air can cause contamination and nonspecific results.
- What is the principle of the Conductimetric method for BUN?
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-Ammonium carbonate is formed after urease treatment
-Conductivity increases - What is measured in the Diacetyl monoxime method for BUN?
- a yellow diazine product
- List 3 different methods for testing Creatinine levels:
-
1. Jaffe'
2. Jaffe'-Kinetic modification
3. Enzymatic - On what reaction are chemical methods for Creatinine determination based?
- Creatinine + picric acid -> orange-red chromagen
- What wavelength is the Jaffe method read at?
- between 490 and 500 nm
- What are interfering substances with the Jaffe method?
- Bilirubin, other nonspecific reactants (glucose/ascorbic acid, etc)
- What reagent can make the Jaffe reaction more specific?
- Lloyd's reagent