microscopy
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- What can be seen via dark field?
- spirochetes
- What can be seen via EM?
- virus and microspordia
- Why is a swab a bad thing?
- 3/100 will grow in culture, anaerobes die, only 150 microL
- What is the order of reagents in gram staining?
- crystal violet, iodine, alcohol, safarin
- What can be stained via Acid-fast?
- mycobacteria, nocardia, cryptosporidia, cyclospora
- What are the reagents for the Acid-fast procedure?
- carbofuchsin red, decolorizer (HCl), counterstain
- What are the special fungal stains?
- lactophenol cotton blue, GMS (gomori methenamine silver, PAS, Mucicarmine
- What does the lactophenol cotton blue stain?
- fungi
- What does Gomori methenamine silver stain?
- yeast, hyphae, pneumocystis carinii
- What does PAS stain?
- yeast calls and hyphae
- What does mucicarmine staine?
- cyrptococcus capsule
- What are silver stains used for?
- bacteria and spirochetes
- What are the two silver stains?
- Warthin-Starry, Steiner
- How are parasites stained?
- Wright's giemsa, wet mounts, trichrome, modified acid fast
- What can the Wright Giemsa stain?
- hitoplasma, leishmania, trypanosomas, malaria
- What is an important carrier of antibiotic resistance?
- conjugal plasmids called R plasmids
- What is a prophage?
- a phage that has entered the genome of the host but remains latent
- What is a lysogen?
- A cell harboring a prophage