Gene Therapy2
Terms
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- Spacer Sequence
- Non-Coding DNA between genes
- Introns
- Non-Coding DNA within genes. Often contain more DNA than exons - make up the majority of DNA sequences in individual genes
- Exons
- Coding DNA sequences
- Untranslated Region
-
Contained in exon but does not code for protein
Regulates translation and transcription
Generally at the end of gene sequences - Primary RNA Transcript
- Contains both exons and introns
- Alternative Splicing
-
Creation of different proteins from the same genes
(ABCD-->ABDC, BDAC, CABD etc)
On average each human gene codes for 3 proteins - Structure of Mitochondrial DNA
- One circular, double-stranded DNA molecule
- Mitchondrial Genome Inheritance
- Sperm contributes only nuclear DNA to zygote, mitochondrial DNA comes from egg
- Whole Shotgun Approach
- Examines similarities in fragments and has computer reassemble based on landmarks and where fragments overlap
- Nested Shotgun Approach
- Orders pieces of sequence first, then blasts it and reassembles based on initial understanding
- Methods to study proteins
-
1-Seperation by 2-D gel electrophoresis using size and charge
2-Study the binding partners or a protein
3-Protein Chip:studying the binding partners
4-Protein structure - How will proteomics help drug R&D?
- Better predicition of drug interactions and efficacy