BIOL2
Terms
undefined, object
copy deck
- The ability to distinguish two points in the specimen (i.e. see two points, instead of one) relates to a problem in using light microscopes termed
- resolution
- The numerical aperature of an objective lens determines the ability of that lens to ________ portions of the specimen.
- discern/view
- A stain is used to induce ______________ in the specimen.
- contrast
- The smallest element in a digital picture is refered to as a
- pixel
- The total magnification through a compound microscope is equal to the magnification of the ___________ times the magnification of the _________________.
- objective lens; eyepiece
- Oil immersion improves light microscopy by increasing the ______ of the medium between the objective lens and the specimen.
- refractive index
- ____________________ refers to the ability to distinguish objects on each side of the plane of focus
- magnification
- A microscopic technique used to increase contrast in an unstained specimen is ________________.
- dark field illumination
- ______________ is the stage of mitosis in which the chromatin material begins to condense into individual chromosomes and the nuclear membrane begins to disappear.
- Prophase
- ______ is the stage of mitosis when the sister chromatids begin to separate to opposite poles
- Anaphase
- _______ is the stage of mitosis in which cytokinesis, the separation of the cytoplasm to the two daughter cells, occurs
- Telophase
- The objective lenses are held in a structure on the microscope known as the ___________.
- revolving nosepiece
- The protozoan you will study in lab this week is ____________
- elodea
- The source of the tissue you will use to study mitosis is ____________________
- onion
- The specimen to be examined with a microscope is placed on what part of the microscope? ______
- stage
- In the lab you will make scrappings of your __________ to look at epithelial cells.
- cheek
- The stain aceto-orcein is used to stain what material inside of cells? __
- DNA
- The lens system located between the specimen and the ocular lens is known as the _____________.
- condenser lens
- What power of objective lens is usually used with oil immersion? _____
- 100X
- Scopes with a single eyepiece said to be monocular. What are scopes with two eyepieces said to be? ________
- binocular
- Which lens is likely to have the largest (longest) working distance? The 10X objective or the 100X objective? ________
- 10X
- The HCl (hydrochloric acid) is used to break down the ____________ of the cells used to study mitosis.
- membrane
- To view the specimen on the microscope, the observer looks through the ___________ lens
- objective
- When you focus your microscope, you change the distance between the objective and the _______
- specimen
- The symbol lambda in Abbe?s equation refers to the ______________ of light
- wavelength
- A difference in the absorption of light by different portions of the specimen is the basis for ___________ (choose ?brightfield? or ?phase?) microscopy
- brightfield
- The illuminator for the microscope is located in the part of the microscope known as the _______
- base
- The angle theta in Abbe?s equation is _______ of the angle of the cone of light entering the microscope objective
- 1/2