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biotechnology

Terms

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DNA fingerprinting
the identificatin of individuals using sections of DNA with little function but that vary wildly
genetic engineering
changing a certain genome, and has been used since the 1970's.
the process of gel electrophoresis
put DNA in wells and put a electric current through the buffer. The DNA fragments will be attracted to the positive end of the gel, as they are negatively charged. Smaller fragments will move faster than larger fragments.
clone
has identical nuclear DNA to another individual
gene therapy
inserting a functional copy of a gene into a cell with faulty copies.
transforming cells
In molecular biology, transformation is the genetic alteration of a cell resulting from the uptake, genomic incorporation, and expression of foreign genetic material (DNA
cloning process
take an egg, take nucleus out of it. take this empty egg and fuse it with a somatic cell. begins to divide and becomes an embryo. implant it into a foster mother.,
PCR
amplifying a single or a few copies of a DNA region, exponentially generating millions or more copies of the DNA piece. There is a higher chance of putting it into your target.
inbreeding
breeding of similar characteristics
restriction enzymes
enzymes that cut the DNA at specific sequences resulting in sticky ends. they are important because they cut the same way on every organism, so that different pieces of DNA can be put together in the way that you want. They are used during genetic engineering.
bioethics
the philosophical study of the ethical controversies brought about by advances in biology
hybridization
cross breeding of dissimilar characteristics
DNA extraction
grinding the cells up, and separating the different components.
human genome project
this project is an attempt to sequence a human's entire genome (all of it's DNA.)
nuclear injection
take a tiny needle and inject DNA straight into the nucleus.
variation
the genetic diversity within a population
transgenic organism
an organism with foreign genes
DNA sequencing
reading the sequences of dna bases along the length of dna (only one strand is read)
genetic markers
anything that has a known locus in the genome, so you can figure out the location of an unknown gene by figuring out how far away an unkown gene is from the known gene.
DNA sequencing process
take four bases and label them with different colors and attach them to the sequence of interest. run the whole thing on a gel and read the different colors.
selective breeding
breeding based on traits that you want to pass on, while not selecting negative characteristics
gel electrophoresis
a procedure used to separate DNA fragments according to size.
sources of variation
mutations, crossing over of the tetrads, meiosis I

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