MicroLab Ex,1,2,3,4,8
Terms
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- Name aceptic tecniques.
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1)bonami soap on slides
2)disinfectent liquid on tables before and after use
3)flame loop and needle before and after use - Explain streak plate for isolation
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purpose: isolate colines and certin type of bacteria
get 1 inoculum then streak, flame, streak, flame, streak, flame - Transfer instraments
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transfer loop
transfer needle - Smear from broth culture
- flame, cool, get loop full of bacteria and wipe on slide in a circulaton motion to make a dime size sample
- Simple stain
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clean w/bonami, then heat dry to take off oil
add smear
heat fix
crystal violet 1min, rince, blot dry - How is the simple stain able to hold on?
- the crystal violet is atracted to the LPS found in Gram - bacteria. The LPS creates a negative net charge out side the cell.
- What are the main cell shapes
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Cocci
Rod
Spiral - Gram stain
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Prepare slide
Crystal violet - primary stain for G(+)
Lugols iodine - mordant
Ethal alcohol - decolorizer
Safranin - counter stain - Compare and contrast G(-) and G(+)
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Gram (-)
thin layer of peptioglycan = thin cell wall
stained red/pink
Outer membrane consists of LPS, lipids, and protiens.
Lipoprotiens help bind the peptidoglycan ot the uter membrane
LPS creates a net negative charge around the cell
Periplasmic space contains hydrolytic and degrading enzymes along with sugars and proteins
Gram positive bacteria are stained purple
No cell membrane
Teichoic and Lipidteichoic acids in the eptidoglycan layer to create a net negative charge around the cell
Major characteristic in both
Plasma membrane/ inner membrane - consists of a phospholipid bilayer and contains proteins - Why expose plate to air
- air has bacteria
- History of Gram Stain
- 1884 Cristian Gram
- Charaeristic of bacteria
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Light transmission - opaque or translucent
Surface - smooth, wrinkled, concentric, contoured
Elevation - raised or convex
Shape - circular, punctiform irregular, rhizoid
Margin - straight, undulate, irregular, filamentous, curled
Odor - earthy, fruity, ammonia
Pigmented - color
Gram stain - Name the shapes of a colony
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filamentous
irregular
round
rhyzoid
curled - Name the edges of a colony
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entire
filamentous
irregular (undulate, lobate) - Name the elevations of a colony
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raised
flat
convet
umbonate
growth int medium