MMG Lab G
Terms
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- What type of surface growth is found in broths?
-
-none
-ring (around edge only)
-pellicle (solid top) - What type of general growth is seen in broths?
-
-diffuse (can't see particles)
-flocculent (can see individual particles like pulp) - What types of growth can be seen in deeps?
-
-surface growth
-partial growth on stab (goes about 1/4-1/2 way down before tapering off)
-complete (equal growth to bottom of stab) - What type of growth is seen on slants?
-
-filiform: one solid growth along line
-beaded: tiny "colonies" along line pattern
-Rhizoid: branching off of lines - What does punctate mean?
- -refers to colonies wich are <.5mm
- How would you describe size for colonies on Agar plates?
-
-punctate <0.5mm
-0.5 mm
-1.0 mm
-1.5 mm
-etc - What are the general shapes of colonies found on Agar plates?
-
-circular
-irregular
-rhizoid
-filamentous
-elliptical -
What are the edges seen on colonies on Agar plates?
How is this best seen? -
-entire (smooth)
-undulate (small waves)
-lobate (amoeba-like lobes)
-erose (like a pointy undulate)
*10X dissecting microscope or hand lens - What are the surface elevations you can see on colonies found on Agar plates?
-
-flat
-raised (concave center)
-convex
-pulvinate (shorter convex)
-umbonate (like a pointy mtn) - What are 4 characteristics used for labeling optical characteristics of colonies on agar plates?
-
-light passing through colony
-reflection of light
-consistency
-pigmentation - What are the 4 characteristics of light passing through a colony on Agar plates?
-
-opalescent: (rainbow colors; mix of opaque and translucent)
-opaque: no light
-translucent: some light, no visibility
-transparent: light and view of agar beneath - What are the two classes of light reflection of colonies on Agar plates?
-
-dull
-glistening - What are the 4 classes of consistency in colonies of Agar plates?
-
-brittle
-butyrous (butter-like)
-membranous (thin growth, coherent)
-viscid (wet looking or mucoid) - What are some colors found in colonies/agar that bacteria produce?
- -white, tan, light yellow, yellow, deep yello, pink, red, blue, green, etc
- What are the three types of solubility that bacteria can exhibit on Agar plates?
-
-water soluble pigments: agar colored
-water insoluble pigments: only colonies colored
-NA if no color is present - How do you measure colonies on Agar plates for FUNGI?
-
-1.0mm
-1.5mm
-2.0mm
-etc - What are the general shapes found on Agar for fungi?
-
-round
-oval
-rhizoid (cloud of spirals)
-filamentous (gaglion) - What are the edges of FUNGI colonies on Agar plates?
-
-entire
-undulate
-erose
-feathery - What are the surface elevations of FUNGI on Agar plates?
-
-flat
-convex
-umbonate
-raised - What are the surface textures for fungi on Agar plates?
-
-filamentous (hairs or fibers)
-folded (gentle rolling hills)
-furrowed (deep grooves and wrinkles)
-glaborous (smooth surface)
-rugate (stomach lining folds)
-wrinkled (brain tissue) - What are the consistencies seen in FUNGAL Agar plates?
-
-cottony (cotton fibers)
-downy (soft feathers)
-fluffy (loose, light, airy mass)
-granular (grainy appearance)
-powdery (crumbly, fine particles)
-velvety (smooth and soft as silk)
-waxy (smooth, soft, pliable surface like wax) - What does pigmentation look like on fungal colonies?
-
-different colors on top from bottom
-range from white to black
-white, tan, peach, salmon, cinn brown, cream, brown, grey, black, etc