Mutagens
Terms
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copy deck
- Ames test
- screening method to determine if a chamical is a carcinogen - cancer.
- After complimentary base pairing
- can now make capsule by exposing to a piece of DNA from the donor
- transformation Grififin 1928
- pathogen --- very heavy capsule (smooth shiny colony) (s) mutant lacked capsule (not pathogen) rough colony (r)
- ionizing radiation
- X-rays, gamma rays, cause DNA damage
- Gene exchange in bacteria 2 events
- 1. donor DNA --- into recipeint cell 2. donor DNA --- into chromosome
- Live R recipient for transformation
- must be in a state of competence log phase, to take up DNA fragments
- Back mutation
- reverse original mutation --- doesnt require histadine as a nutrient, can make its own
- Forward mutations
- mutant can not make histadine requires histadine as a nutrient
- impersonate chemical mutagen
- impersonate N - base incorporated into DNA leads to faculty pairing
- Radiations
- UV - structural changes in the DNA
- chemical mutagens
- combine with N - base - alter them so that they mispair Ex - Nitrous acid - combines A causes it to pair with cytosine