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biochem 2

Terms

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Dansyl chloride or FDNB aromatic     
renders AA visible in spectrophotometry
Phenyliosthiocyanate (PITC)
used to form PTH derivatives of AA
Cyanogen Bromide
cleaves peptide backbone, forms homoserine lactone
Chymotrypsin   
cleaves at Try, Phe, and Trp
Iodoacetate
acetylates SH so disulfide bond cannot reform
6 M HCL
USed to prepare protiens for AA compostion
Dithiothreitol (DTT)
removes Cys residues, reduces S-S
Hydrophobic force  
Two nonpolar AA
H bonding force
Polar and a negative AA, like OH and COO-
Ionic force
a + and a -
Dipole-dipole force
two polar, uncharged
covalent force
look for the amide
Proton donor
lewis acid, NH+
Proton acceptor
lewis base, N:
BPG down
O affinity up
BPG up
O affinity down
pH up
O affinity up
pH down
O affinity down
PO2 up
O affinity down
PO2 down
O affinity up
During O bonding, iron is in what form?
Fe2+
Before O bonding, iron is in what form?
Fe2+
the alpha and beta chains interact more or less after bonding?
less
during O bonding, the binding curve gets more
sigmosidal
AA inside helix are
non polar
AA outside helix are
polar
Consecutive AA stabalize helix when they are
of opposite charge
AA interacting in helix need to be how far apart in the sequence?

4 AA

X 1 2 3 Y 

increasing [E] does what to Km
nothing
decreasing [E] does what to Km
nothing
increasing [E] does what to Vmax
increases propotionally
decreasing [E] does what to Vmax
decreases proportionally
Km is better when
lower
Km is

k-1 + k2 / k1

 

ES destruction/ ES formation 

Vmax is

K2[Et]

rate of product formation times all enzyme concentration. 

Kcat is
Vmax/[E]
Turnover number is
Vmax/[E]
Catalytic efficiency is
Kcat/Km
specifity constant is
Turnover # / Km
NH+ is a
Nucleophile or lewis acid
N: is a
electrophile, or lewis base

Deck Info

41

leficent

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