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Non-fermenting GNB 2


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What do ALL fluorescent bacteria produce?
what does Pseudomonas aeruginosa produce?
Pyoverdin and Pyocianin
-Bluish-green on clear agar
-Metallic on blood agar
What 4 characteristics help to identify P aeruginosa?
-Oxidase pos
-Grape odor
What are the members of the Pseudomonas group?
P aeruginosa
P fluorescens
P putida
P stutzeri
What is important to know about Pseudomallei group in prescribing antibiotics?
ALL resist
-Polymyxin B and Colistin
-were previously Pseudomonas but now are Burkholderia.
What are the three members of the Pseudomallei group?
1. Burkholderia cepacia
2. Burkholderia pseudomallei
3. Burkholderia mallei
-Which of the pseudomallei are u most likely to see?
-What does it cause?
Burkholderia cepacia - causes chronic pneumonia in cystic fibrosis patients.
How are Burkholderia pseudomallei/mallei acquired?
From inhalation from animals.
What does Burkholderia pseudomallei cause?
Where is it seen?
Melioidosis - a febrile septicemia in SE Asia.
**Handle in safety cabinet!**
What does Burkholderia mallei cause?
GLANDERS neieiiiiighhhh.
The common nonfermentors are:
#1=Pseudomonas aeruginosa
#2=Acinetobacter baumannii
#3=Stenotrophomonas maltophilia
What are distinctive characteristics of Stenotrophomonas?
-Smells like ammonia!
-Oxidizes Maltose/Glucose
What is the distinctive characteristic of Shewanella putrefaciens?
-Only NF GNB to produce H2S
What are the 2 species of acinetobacter?
Acinetobacter lwoffii
Acinetobacter baumannii
What distinguishes both acinetobacter species?
-Oxidase neg
Which 3 NF GNB are normal flora and where?
-Acinetobacter - vagina
-Moraxella - mouth/GI tract
-Eikenella - mouth/GI tract
How are the acinetobacters different?
Baumannii oxidizes glucose
Lwoffi doesn't oxidize at all
Are moraxella cocci?
NO; they are true bacilli, but coccobacilli.
What is the yellow nf GNB?
Chryseobacterium meningosepticum
Where does Eikenella corrodens often cause infections?
in human bite wounds b/c it's normal flora in mouth.
what does c. meningosepticum cause?
nosocomial neonatal meningitis.
Which 2 nf GNB are oxidase neg?
Which nf GNB don't oxidize glucose?
-bordetella bronchiseptica
-eikenella corrodens
acronym for the non glucose oxidizers:
morax, alcal, bordatll, eik,
Which nf GNB oxidize maltose?
1. Ps. stutzeri
2. Burkholderia cepacia
3. Stenotrophomonas
4. Chyrseobacterium meningos.
(Acinetobacter baum. varies)
Which species doesn't do ANYTHING? Except...
Acinetobacter lwoffi - only grows on MAC and is GNCB
Which nf GNB are coccobacilli?
Which nf GNB oxidize lactose?
-Burkholderia cepacia
-Stenotrophomonas MIGHT
which nf GNB does EVERYTHING? what doesn't it do?
Burkholderia cepacia
-no specific morphology.
Which nf GNB doesn't grow on MAC?
-Eikenella corrodens
-Moraxella is variable.
Which 5 nf GNB are NOT motile?
-Chryseobacterium mngsptcm
-Eikenella corrodens
what acronym says which are nonmotile?
Which pseudomonas is green?
Which pseudomonas is yellow?
which pseudomonas is wrinkly?
How do you tell P. putida and stutzeri apart?
Putida = gelatin +
Stutzeri = gelatin -
Which nf gnb is tan and smells like ammonia?
-Oxidase pos
-Neg carbs (all)
-Growth on MAC
-Oxidase neg
-Neg carbs (all)
-Growth on MAC
Acinetobacter iwoffi
-Oxidase pos
-Neg carbs (all)
-Growth on MAC
What has an Earthy odor and
Peritrichous flagella
What is pos oxidase and everything except motility?
Chyseobacterium meningosepticum
How do you seperate Bordatella bronchiseptica from Eikenella?
-Both are pos oxidase
-Both neg on all carbs
-Ekenella doesn't grow on MAC
-Eikenella isn't motile
Which nf GNB is urea pos?
Bordetella bronchiseptica
Which nf GNB is yellow?
Chryseobacterium meningosepticum

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