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Microbiology - misc gram neg rods


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Diseases caused by Haemophilus influenzae
Prior vac: a. meningitis <6 yrs, bacterial conjunctivitis, bacterial epiglottitis, otitis media in children, acute sinusitis
also: bronchitis, cellulitis, pneumonia
transmitted via respir. route
Identification of H. influenzae
Pleomorphic GRAM NEG RODS
requires X&V factor (choc agar)
tests NEG for porphyrin (ability to synthesize hemin from d-ALA)
predominant disease manifested by H.influenzae biogroup aegyptus
causes pink eye
predominant disease manifested by H. ducreyi
agent of chancroid (soft chancre) - ulcer on genitalia
main characteristic about H. parainfluenzae
normal flora of upper respiratory tract
characteristics of Bordetella pertusis
Causative agent for Whooping cough
trnsmtd via respir droplets, organism colonizes mucous mbrns of resp tract
affinity for epithelial cells of nsophrynx & elaborates exotoxin
Disease of infants/children, but now adults
Prvntn through active immunizations
Identification of Bordetella pertussis
Colonies look like MERCURY DROPLETS
Gram NEGATIVE rods/coccobacilli - small
Direct Fluorescent Ab (DFA) stain
Disease of Legionella pneumophila
"chance" infection thru H2O distribution systems. no known p2p transmission
Serious pneumonia w/ involv of kidneys, heart & rectum
Risk factors: smoking, drinking, underlying illness (diabetes or immunosuppresion), more in males
Identification of Legionella pneumophila
DFA stain
DNA probing
detection in the urine
3 major Brucella species
B. abortus - man & cattle
B. melitensis - man & ruminants
B. suis - man & pigs
What is zoonoses?
A disease transmitted to humans from animals
What is undulant fever?
Caused by Brucella spp.
ingestion of unpasturized milk, direct contact w/ animals, inhalation of particles
Chills, fever, sweating, weakness, fatigue, weight loss
Identifying Brucella spp
gram NEGATIVE coccobacilli
ID to species level - send to ref lab
main animal reservoir for Francisella tularensis

is virulent for most mammals
How is F. tularensis transmitted to humans?
direct cutaneous inoculation or from tick/deerfly. Can also be from inhalation and ingestion
what is special about virulence of F. tularensis in terms of amt needed for infection?
highly infectious - only 10-40 bugs needed for infection
Culture and isolation of Francisella tularensis
Use Glucose Cysteine blood agar, choc w/ isovitalex, or modified charcoal-yease extract
incubate 35C w/ or w/o CO2
ID: send to ref lab, gram NEGATIVE coccobacillus w/ bipolar staining, serology
Char of Pasturella multocida
Assoc w/ animals; normal flora in cats, dogs, rabbits, panthers, etc
Common pathogen in dog/cat bites or scratches: cellulitis develops
Use penicillin to treat
ID'ing Pasturella multocida
Grows well on SBA & Choc
NO GROWTH on MacConkey
Gram NEGATIVE pleiomorphic coccobacillus

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