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Comparison of Immunoassays


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What are the 5 advantages of latex agglutination?
1. versatile
2. Rapid
3. no equipment needed
4. Good screening test
5. Long-lived reagents
What are the 2 drawbacks of latex agglutination?
1. Variability btwn manufacturs
2. Not quantitative.
What are the 3 advantages of hemagg. inhibition?
1. Reference methodology
2. Good for IgM assays
3. Easy to read
what are the 2 drawbacks to hemagglutination inhibition?
1. Time consuming
2. Varied sensitivity
what are the 2 advantages of EMIT?
1. Simple to perform
2. No separation step needed
what are 2 drawbacks of emit?
1. Limited to low MW antigens
2. Not as sensitive as other EIAs
what are the qualities of ELISA?
-long-living reagents
-variety of detection systems
-good for automation
-adaptable for screening
-qualitative OR quantitative
what are the drawbacks of ELISA?
only cost-effective for batching
-variability of SP carriers
-some agents are carcinogenic or expensive
what are the advantages to direct or indirect IFA?
-relatively inexpensive
-long-lived reagents
-variety of methods
-sameday results for DFA
what are the drawbacks to IFA?
-background/nonspcfc fluorscn
-time consuming
-interpretation needs expert
what is the advantage of RIA?
-Disposal of reagents
what are the advantages of SPRIA and IRMA?
-More stable/sensitive/rapid than plain RIA
-measures total amt of antibody
-uses less reagent than RIA
what are the drawbacks of SPRIA and IRMA?
same as RIA - disposal/cost
what are the advantages of complement fixation?
1. Reference method
2. Relatively sensitive
3. Adaptable to many tests
what are the drawbacks to complement fixation?
-time consuming
-requires many controls
-expensive reagents

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