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CLLS124: Staph


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What is the catalase reaction for Staphylococcus?
Which tests which differentiate between S. aureus and the Coagulase negative Staphylococci
Thermostable nuclease test
Identify this bacterium:
catalase positive
Staphylatex test positive
Staphylococcus aureus
What test will differentiate between S. saprophyticus and other Staphylococci?
Novobiocin resistance

S. saprophyticus is resistant to novobiocin; other Staphylococci are susceptible.
What is the DNAse reaction of Staphylococcus epidermidis?

Of the Staphylococci, only S. aureus is positive
What type of coagulase is detected by the slide coagulase test?
Bound coagulase.

The tube test detects both free and bound coagulase.
Describe the principle of the Staphylatex test.
Latex beads are coated with fibrinogen and anti-Protein A.

S. aureus has coagulase which converts the fibrinogen to fibrin, forming a clot. It also has the Protein A antigen -- when the antibody reacts with the antibody you get agglutination. Overall, a positive reaction is clumping.
How does Micrococcus compare to Staphylococcus?
Both are gpc, catalase positive.
Micrococcus has a brighter yellow pigment.
How does Stomatococcus compare to Staphylococcus?
Both are gpc.
Staphylococcus is catalase positive; Stomatococcus is catalase variable.
Stomatococcus colonies are sticky as compared to Staphylococcus.
Where are these bacteria normal flora?
a. Staphylococcus aureus
b. Coagulase negative Staphylococcus
c. Stomatococcus
a and b. skin and mucus membranes (mouth and vagina)

c. mouth
What type of infection is associated with Staphylococcus epidermidis?
SBE when a prosthetic heart valve is involved.

Bloodstream infections
Name diseases associated with S. aureus.
Skin diseases (e.g. acne, folliculitis, impetigo, boils, scalded skin syndrome)
toxic shock syndrome
staph food poisoning
wound infections
What infection does S. saprophyticus cause?
UTI in sexually active 18-25 year old females
What is the first choice for antibiotic treatment of S. aureus?

If methicillin resistant, use vancomycin.

For VISA, GISA, VRSA strains, use Synercid or Zyvox.

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