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Biochem1011

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Key Enzyme(s):

Glycogenolysis

glycogen --> glucose

*Glycogen phosphorylase

Key Enzyme(s):

Glycogenesis

glucose --> glycogen

* hexokinase
* glycogen synthase


Key Enzyme(s):

Glycolysis

glucose-6-P --> pyruvate

* PFK
* pyruvate kinase


Key Enzyme(s):

Gluconeogenesis

pyruvate --> glucose

* pyruvate carboxylase
* PEPCK
* F-1,6-bisphosphatase
* glucose-6-phosphatase




Key Enzyme(s):

Pyruvate --> acetyl CoA

* PDH
Key Enzyme(s):

TCA cycle

* isocitrate DH
* alpha ketoglutarate dehydrogenase
* succinyl CoA synthase

Key Enzyme(s):

Fatty Acid Synthesis

acetyl CoA --> fatty acid

* acetyl CoA carboxylase

Key Enzyme(s):

Lipolysis

triglyceride --> glycerol + FA

* hormone-sensitive lipase

Key Enzyme(s):

Lipogenesis

glycerol + FA --> triglyceride

* acyltransferase

Key Enzyme(s):

Amino Acid Oxidation



AA --> glutamate -*-> NH3 + alpha Kg

* glutamate DH

NH3 -**-> urea

** CPS





Key Enzyme(s):

Fatty Acid Oxidation

Fatty acyl CoA -*-> fatty acylcarnitine --> (mitochondria) --> acetyl CoA

* CPT1 ...(CAT1?)

3 Sources that lead to Glucose-6-Phosphatase production
1. hexokinase (glucokinase)
2. gluconeogenesis
3. glycogenolysis (glycogen breakdown)

4 Fates of glucose-6-phosphatase
1. glycolysis
2. pentose phosphate pathway
3. glycogenesis (glycogen synthesis)
4. free glucose


3 Sources of Pyruvate
1. lactate
2. alanine
3. glycolysis

5 Fates of Pyruvate
1. alanine
2. lactate
3. oxalacetate
4. glucose
5. acetyl CoA



4 Sources of Acetyl CoA
1. pyruvate
2. amino acids
3. ketone bodies
4. fatty acids


4 Fates of Acetyl CoA
1. ketone bodies
2. amino acids
3. fatty acids
4. cholesterol


Excess NADH and absence of QH2
poisoned by amytal or rotenone (complex I)
If the mitochondria are still consuming oxygen..
poisoned by 2,4 DNP (uncoupler)
There is an excess of QH2 and an absence of reduced Cyt C, and the mitochondria are not still consuming oxygen
poisoned by Antimycin (complex III)
Coenzyme A
2 carbon carrier

Pantothenic Acid, B5

TPP
2 carbon carrier

thiamine, B1

NAD
hydrogen carrier

niacin, B3

FAD
hydrogen carrier

riboflavin, B2

Lipoic acid
2 carbonr carrier +/or H

Not a vitamin source

Tay-Sachs Disease
Enzyme deficiency: hexoaminidase A
Niemann-Pick disease
enzyme defeciency: sphingomyelinase

*liver and spleen enlargement

A-I
Activator of LCAT (HDLs)
B-100
Synthesized in liver

ligand for LDL receptor

C-II
Activator of extrahepatic lipoprotein lipase
D
possibly identical to the cholesterol ester transfer protein

Deck Info

31

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