Glossary of vibrio, campy, helicopter
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- species of vibrio
- Vibrio cholerae
- what disease does vibrio cholerae cause?
how is it carried around
- cholera; it's a waterborne pathogen
- what does vibrio genus like? (environment)
- warm seawater
- shape of vibrio
- curved, like a COMMA
- What is the major pathogenic characteristic of V. cholerae?
-What 2 main symptoms result?
- Exotoxin, aka enterotoxin.
1. Diarrhea (rice water stools)
2. Severe dehydration
- symptoms of cholera
- diarrhea (rice water stools)
- What 4 features are used for diagnosing V. cholerae?
- 1. Curved gnb
2. Growth/color on TCBS agar
3. Biochemical sugars
4. String reaction
- 4 points included in lab diagnosis of V. cholerae:
- -if we know it's a curvy gnbacillus
-if it's hard to grow on normal enteric media, but grows well on TCBS.
- What is the purpose of TCBS agar?
- Allowing growth and differentiation of Vibrios; inhibiting enteric bugs.
- what sugars does v. cholera ferment?
- all of 'em: gluc, lact, and sucr
- what's the string reaction?
- when you mix vibrio cholerae with .5% sodium desoxycholate it makes a mucousy string. not very technical tho
- which vibrio is associated with shellfish/raw fish/seafood and in the far East?
- vibrio parahemolyticus
- what are the symptoms of vibrio parahemolyticus?
- Enteritis from shellfish; self-limiting, but severe sometimes bloody diahrrea.
- Which two vibrios are halophilic
How is this feature used to identify them?
- Parahemolytic and Vulnificus
-Makes them turn green-blue on TCBS agar.
- what does NaCl do to vibrio cholera?
- inhibits its growth.
- how many other species of vibrio are there? characteristics?
- 9; nonpathogenic; oxidase positive GNB
- What disease is caused by Vibrio vulnificus:
Wound/ear infections following exposure to seawater.
- List 4 basic characteristics of Campylobacter:
- 1. GNBacillus - CURVED like S
2. oxidase +
3. obligate microaerophile (blw srfc)
4. non-fermenter - no carbs
- what is the main pathogen of the campylobacters?
- campylobacter jejuni
- -How is C.jejuni transmitted?
-What disease/sx does it cause?
- -Via ingestine of uncooked poultry.
-ENTERITIS: diahrrea, fever, bloody stools
- 2 Virulence factors produced by campylobacter jejuni:
- selective media for campylobacter: contains:
- campy BA
brucella agar base + blood + antibiotics
inhibits normal flora from intestines
- what are the conditions necess for incubating campylobacter jejuni?
- 42 degrees C
- What 5 features of Campylobacter jejuni allow presumptive ID?
- Gram stain = gnb, curvy
Oxidase = positive
Catalase = positive
Motility = darting
- what bacteria is campylobacter jejuni very similar to?
- e. coli
both cause diahhrea
have similar biochemistries
treat w/ same drug
- what is campylobacter fetus ss. fetus?
what does it cause?
- a subspecies of campylobacter;
causes septicemia, is a very rare pathogen;
- what bug is know for causing gastritis and peptic ulcers?
- helicobacter pylori
- what does helicobacter pylori prolificly produce?
- urease; causes gastritis and peptic ulcers.
- what type of flagella does helicobacter have?
- 3 specimens used to identify helicobacter pylori:
-gastric mucosal biopsy
- what 3 key biochemical rxtns of helicobacter pylori allow rapid id?
-All three are POSITIVE
- what is helicobacter pylori treated with?
- ampicillin and bismuth salicylate
- Strategy of Helicobacter pylori I.D.:
- -endoscopy - biopsy specimen
-histology w/ special stains
-culture the bug
-PCR to i.d. nucleic acid
-Serology to test for Ab in serum or whole blood.
- how does the urea breath test work? what organism is it for?
- helicobacter pylori.
the bug produces urease; give the host urea w/ labeled C; if the bug is there, it'll break down the urea and labeled CO2 will be exhaled.
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