Glossary of replication
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- what functions does DNA polymerase do
- -maintains accuracy
-creates phosphodiester bond
- requirements of DNA enzymatic synthesis:
- -4 activated precursors (dNTP's ACGT)
- in the replication reaction mechanism which phosphate residue does the 3' hydroxyl attack
- how is back reaction eliminated in replication
- H2O hydrolyzes PPi to 2 Pi
- Which direction does DNA polymerase move along the DNA template, and which direction is the new DNA molecule synthesized?
- Moves along template 3'-5'
Synthesizes in 5'-3' direction
- Kornberg enzyme is aka
- DNA Polymerase I
- What are the three activities of DNA polymerase I
- DNA Polymerase
- What is the function of 3' exonuclease in DNA Polymerase I
- to remove unpaired bases added to the 3' (hydroxyl) end of growing chain.
PROOFREADS - excises incorrect base pairings.
- how is the lagging strand synthesized in DNA replication
- in okazaki fragments
short 3'-5' pieces
- what enzyme synthesizes primers for DNA replication
- What enzyme elongates primers
- polymerase 3
- what kind of polymerase is PRimase
- an RNA polymerase
actually synthesizes short RNA's as primers.
- Function of DNA polymerase 1 in dna replication:
How does it do its function?
- to remove RNA primers from the molecule.
Binds to 3' hydroxyl of okazaki fragments; sythesizes DNA while its 5' exonuclease hydrolyzes the RNA primer one nucleotide at a time. UNTIL 1 NICK is left.
- what seals the last little nick in DNA during replication
- DNA ligase
- what supplies energy for DNA LIGASE to seal the last nick
- ATP or NAD hydrolysis
- funciton of GYRASE in transcription:
-what does it require
- introduces NEGATIVE supercoils downstream of replication site to counteract gyrating from twisting as DNA unwinds.
-Requires one ATP per supercoil
- What does Helicase do and how?
- -Binds to lagging strand at fork to seperate the DNA double helix so two new strands can be synthesized.
Hydrolizes ATP for energy as leading strand advances.
- WHAT DO SSB PROTEINS DO
- bind to lagging strand in wake of helicase
- How many subunits on DNA Polyerase 3?
- 17, arranged in four subassemblies.
- the names of the four subassemblies on DNA polymerase 3:
- 1. core polymerase
2. beta clamp
3. clamp loader
4. tau dimer
- funciton of B-clamp
- binds to the core polymerase (site of enzyme activity) and holds it onto DNA template strand.
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