Glossary of haemophilus y bordatella
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- gram stain of haemophilus
- pleomorphic gnb
- oxidase of haemophilus
- variable; not useful to id it
- 2 growth requirements of haemophilus
- X factor - hemin; hemoglobin.
V factor - NAD
take advantage of these in a Porphryin test to id the bug
- types of media grow haemophil on:
- -Choc/horse/rabbit BA
NOT ON SHEEPS! CAUSES SATELLITISM
- Why can't you use sheeps ba?
- produces NADase
s. aureus produce NAD; the reaction causes haemophilus to spread out and it's difficult to isolate then.
- how can you differentiate species of haemophil?
- -different types of hemolysis
-different growth requirements (only X, only V, both..)
- type of differential plate for species of haemophil:
- QUAD plate
-X and V
- where is haemophilus normally found
- throat in sm amounts
- virulence factors of haemophilus flue
- What are three infection types caused by H. influenzae?
What are the four resp tract infections?
- 1. Respiratory
Respiratory tract infections:
Sinusitis, Otitis media, Pneumonia, Epiglottitis (acute). SOPE
- What are 2 infections caused by haemophilus species (not just plain influenzae)?
- Meningitis: caused by H. influenzae B in kids <2yrs.
Conjunctivitis: pink eye caused by H. aegyptius.
- how do you treat haemophilus, and what test should you do first?
+ = give cloramphenicol if its warrented (by a systemic infection)
- = give ampicillin
- what can you prevent haemophilus flue with?
- hib vaccine for infants under 2
- where are the # other Haemophilus species found normally, and what do they cause in who?
- 7 species; these are VERY COMMON in the oral cavity.
May cause disease in immunodef patients
-bacteremia, SBE, occasionally pneumonia
- What causes Chancroid?
What symptoms are seen in this?
What is distinct on the gram stain of this species?
- -Haemophilus ducreyi
Gram stain = RRtrax of gnb's.
- What is chancroid?
- An STD w/ genital lesions softer than those in syphilus.
- GRAM stain of bordetella
- what does bordatella pertussis cause?
specimen of choice?
- whooping cough
- 3 types of media to grow bordatella on
- -Regan Lowe (transport)
-Bordet Gengou (mercury droplet colony morphology)
-Charcoal Agar w/ 10% horse blood + cephalexin
- Environmental conditions necessary for growing Bordatella pertussis:
- 72 hrs in increased CO2; its fastidious
- 2 methods of identification of B. pertussis:
- 1. On specimen: Direct Flourescent Antibody
2. On growth:
- What is DFA, on what bug is it useful for identification?
- Direct Flourescent Antibody testing on bordatella pertussis;
-label Ab w/ flourescent tag
-complex to antigen
-Look for flouresc. w/ microscope
- whooping cough aka
- How is whooping cough transmitted?
- through droplets or direct contact
- 3 stages of pertussis
- What does the Catarrhal stage consist of?
- 2 wks of typical cold symptoms.
-The Most contagious stage.
- Paroxysmal stage
- 2nd Pertussis stage;
-Lasts 2 weeks;
-Intensified cold symptoms;
-More serious/distinct WHOOPING/gasping cough
- Convalescence Stage
- 3rd Pertussis stage;
Lasts 2 more weeks;
A Gradual relenting of symptoms/coughing.
- What antibiotic is used to treat Pertussis?
- How can pertussis be prevented?
- With the DPT (diptheroid, pertussis, bla)
only in people younger than 7
- What infection is caused by Bordatella parapertussis?
- Resp inf milder than whooping cough; no vaccine, usually just thought to be a cold
- How does culturing Bordatella bronchiseptica compare to the other bordatella species?
What test can rapidly id it?
What 3 diseases does it causes?
- Nonfastidious, this bug grows well on BA and MAC.
Urea test -> rapidly positive!
resp tract/wound infections/pneumoniae
- things we can know about VIBRIO (all three) in general:
- oxidase positive
gnb CURVED like a COMMA
like warm seawater
- XV requirements, hemolysis of H. influenzae:
- X and V
- XV requirements, hemolysis of H. hemolyticus:
- X and V
- XV requirements, hemolysis of H. parahemolyticus:
- V only
- XV requirements, hemolysis of H. parainfluenzae:
- V only
- XV requirements, hemolysis of H. paraphrophilus:
- V only
- XV requirements, hemolysis of H. segnis
- V only
Mannose neg -
- XV requirements, hemolysis of Haemophilus aphrophilus
- X only
- XV requirements, hemolysis of Haemphilus ducreyi
- X only
Gamma or Beta
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