Glossary of component prep
Other Decks By This User
- What are the 4 anticoagulants used in preparing RBCs?
- 1. ACD (acid citrate dext)
2. CPD (citrate phosph dext)
3. CP2D (cit-phosph-doubdext)
- What are the storage times of each anticoagulant?
What temp is indicated?
- ACD: 21 days
CPD: 21 days
CP2D: 21 days
CPDA-1: 35 days
All: 1-6 degrees C
- What 3 additive solutions are used for RBCs?
- 1. Adsol
- What are the storage times for additive solutions?
- 42 days
- Why is CPD better than ACD?
- 2,3-DPG levels lower in 1st week with ACD; they lower in the 2nd week with CPD.
- How does CPDA-1 enhance storage?
- Adenine drives glycolysis to make more ATP and lengthens RBC survival.
- What is the purpose of additive solutions?
- -Preserve packed RBCs that have no plasma.
-Decrease viscosity of unit
- What 5 changes occur in blood with storage?
- -pH decrease
-Glucose consumption decrease
-Lactic acid buildup
-Reversible 2,3-DPG decrease
- What is the shelf life of RBCs or WBCs in:
- ACD: 21 d CPD: 21 d
CPDA-1: 35 d AS: 42 d
- What is the shelf life of irradiated RBCs or WB?
- Original outdate or 28 days, whichever comes first.
- What is the shelf life of an OPEN system of RBCs or WB?
- 24 hrs, if at 1-6'C
4 hrs if at RT
- What is the shelf life of washed RBCs?
- 24 hrs
- What is the shelf life of frozen RBCs?
- 10 yrs
- What is the shelf life of any platelet unit?
- 5 days
- What is the shelf life of pooled platelets?
- 4 hrs - they have to be opened
- What is the shelf life of FFP at
- -18: 1 yr
-65: 7 yr
- What is the shelf life of PF24?
- same as FFP
- What is the shelf life of Cryo?
- Frozen: 1 yr
Thawed: 6 hrs
Pooled: 4 hrs
- What temp must cryo be thawed at?
- What is the shelf life of WBCs?
- 24 hrs
- What is the shelf life of PPF (plasma protein fraction)?
- 5 yrs
- What is the indication for WB? What is the standard vol?
- Volume expansion; 450-500 ml
- What is the indication for irradiation?
- To prevent GVHD
- What is the indication for RBCs? What is the standard vol?
- To increase O2; anemia.
- What is the indication for leukoreduced RBCs?
- To prevent febrile reactions
- What is the indication for Washed RBCs?
- IgA Negative persons, PNH
- What is the purpose of frozen RBCs?
- Rare phenotypes
- What is the indication for Platelets?
- Thrombocytopenia, DIC, bleeding.
- What is the indication for single donor platelets?
- Platelet refractoriness (reaction to HLA on platelets)
- What are the volumes of:
-Random donor platelets
-Single donor platelets
- RD: 50-70 ml
SD: 200-400 ml
- What transfusion criteria applies to SD platelets that doesn't to RD?
- Must be HLA compatible
- What is the storage temp of all platelets?
- What is the indication for FFP?
- Coagulopathy, liver disease, DIC, massive transfusion.
- What is the typical vol of FFP?
- 200-250 ml
- What is the indication for Cryoprecipitate?
- -Hemophilia A
-Fx Eight deficiency
-Fibrin sealant, hypofibrinogen
- What is the typical volume of cryoprecipitate?
- 10-25 mL
- What is teh typical indication for WBCs?
- Neutropenia < 500 cells/uL
- What is the indication for PPF?
- Plasma volume expansion
- Which components must be ABO compatible?
- WB, RBCs, FFP, WBCs.
- Which components do not have to be ABO compatible?
- Platlets, Factor concentrates, and Albumin.
- What are the physiological requirements of a blood donor?
- At least 110 lbs, pulse between 50-100 bpm, Hct at least 38%
- What test result for Hep B defers a donor?
- Pos HBsAg since 11th bday
- When does malaria history disqualify a donor?
- If treated in past 3 years
- What 2 circumstances defer a donor for one year?
- -Blood transfusion
-Positive syphilis serologic test
- When does aspirin disqualify a donor?
- If attempting to give platelet pheresis, can't take within 3 days prior to donation.
- How much time must elapse between consecutive blood donations?
- 8 weeks.
- What permanently defers a blood donor?
- -Hemophilia A/B history
-von Willebrand's Disease
-Pos HIV test
- How long is a donor deferred if received live viral vaccines?
- 2 weeks
- Which vaccines carry a 4 week deferral?
- -German measles (rubella)
- What are the physiologic requirements for autologous donation?
- Hgb of 11g/dL
Hct of 33% or higher
- Quick; Storage temps are:
- WB = 1-6'C
Plt = 20-24'C
- What 7 tests must be done on donor units?
- 1. Syphilis
2. Anti-HIV 1/2
3. HIV antigen
4. Anti-HTLV 1/2
- What is the required Hct of PRC?
- Not >80%
- What is the new expiration date of irradiated products?
- 28 days, unless the anticoag used was only 21 days anyway.
- What is the max level of WBCs in leukoreduced RBCs?
- 5 x 10^6
- What is the platelt count in:
-Random donor units?
-Single donor units?
- RD: 5.5 x 10^10
SD: 3.0 x 10^11
- How soon does FFP have to be prepared after collection?
- Within 8 hrs
- What is Cryo prepared from?
What does it contain?
Fibrinogen and Platelet Factors
- What is the purpose of buffy poor units?
- To leukoreduce a unit and prevent anyphylctic reaction in IgA deficient or PNH patients.
- What are the time limits for preparing:
- Platelets: within 6 hrs
FFP: within 8 hrs
Cryo: within 6 hrs of thawing, 4 hours if ffp is pooled.
- What populations would be eligible for CMV-neg units?
- -Pregnant women
-Neonates, premature infants
- Which blood components are at risk for CMV transmission?
- RBCs and platelets
- When CMV neg units are not available, what is an appropriate alternative?
You must Login or Register to add cards