chpt 8
Terms
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- antigen processing
- degradation of protein antigen into peptides by sequence of everts
- type of antigens that mhc 1 - binding fragments are derived from
- ENDOGENOUS
- PATHWAY that endogenous proteins go through to be degraded/presented
- cytosolic
- type of antigen from which mhc 2-binding fragments are derived
- exogenous
- self-mhc restriction
- an attribute that allows both types of T cells to recognize Ag only if its presented on SELF mhc
- experiment to prove self-mhc restriction
-
1. incubate macrophages w/ antigen
2. Mix pulsed cells with T cells from Same, Different, or Heterogen. animals.
Same = activated
Heterogen = activated
Different = no activation -
Target cells
APC -
-cells w/ peptide display assoc. w/ MHC1 and CD8+ Tc cells
-cells w/ peptide display assoc. w/ MHC2 and CD4+ Th cells - distinguishing feat. of APC
-
-express MHC2
-deliver co-stim signal - Professional Ag-presenting Cells:
- Dendritic, Macrophages, B lymphocytes
- Dendritic cells
-
most effective APCs
-constitutive high expression of MHC2
-can activate NAIVE Th - baseline level of MHC expression in APCs
-
dendritic = HIGH, costim active too
macrophages = low, need both activated
B cells = higher, but need costim activation - what cells function as TARGET cells
- nearly all nucleated cells
- proteasome
- multifunctional protease complex that can degrade ubiquitin-protein conjugates (proteins labeled for degradation)
- increased levels of which interferon induces proteasome subunits? FOR which MHC do the subunits generate antigen fragments? which pathway of degradation is this?
-
-IFN-gamma
-MHC1
-cytosolic pathway - chemically, what type of residues are on the ends of protein fragments that bind MHC 1?
- basic or hydrophobic terminal residues.