Glossary of automated counters
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- What machine measures MCV and calculates the hematocrit?
- What machine measures Hct and calculates the MCV?
- which analyzer does not use light scatter?
- Which analyzer has to manipulate eos/basos to differentiate them?
- which analyzer has von behren's plates instead of sweepflow?
- cell dyn
- what is the principle of the cell dyn?
- cells are passed between plates with apertures of different size; prefer to flow through the larger, and get counted.
- which analyzer uses DC/RF for volume determination and differentiation?
- is there a chance of coincidence loss on the cell dyne?
- yes; to correct, use pulse editing.
- which analyzers seperate a section of cells for hemoglobin via cyanmethemoglobin colorimetry?
- all of them
- what two ways are white cells counted and differentiated on the cell dyne? (2 acronyms)
- WIC - white cell impedence cnt.
WOC - white cell optical count.
the first uses impedence, the second uses light scatter.
- what is different between the sysmex 9000 and 3000?
- 9000 uses DC/RF; the 3000 does not use RF for differentiation, but instead light scattering.
- what is cell dyn's claim to faim?
- WIC and WOC - two counts based on impedence, and light scatter.
- what angles does the cell dyn scatter light?
- FAS - forward
orthogonal - 90, right angle
10 - narrow angle
- On Celldyn, what does the FAS measurement tell you?
-90 right angle?
- FAS = the size of cell
right angle = granularity
depolarized = eosinophils
10 = n:c ratio - nucleus density
- what type of sheath fluid is in the cell-dyn?
- weak salt that pulls hemoglobin out of cells so there's no interference.
- what type of light source is used in the cell-dyn?
- polarized helium argon laser.
- what is the acronym for the special light scattering used in the cell dyn?
- MAPSS - multi angle polarized scatter seperation
- what is list-mode analysis?
- whatever electrical information is gathered from each cell is given in a direct plot, not a box scatterplot.
- which analyzer shows exactly whre basophiles are in the electrical information?
- cell-dyn, don't have to rotate the scattergram to visualize them.
- what is advia technology based on?
what is it NOT based on?
- light scattering
not on electrical impedance.
- is advia good?
what is it good at?
- no; it's unwieldy and difficult to interpret;
give the best RBC information
- What do cold agglutinins do to an analysis?
- cause RBCs to clump and give an abnormal RBC count;
Hct will be wrong, but Hgb will be normal because cells are lysed.
- how do you fix cold agglutinins?
- warming the sample and rerun it.
- What causes a high take-off?
- anything smaller than a lymph.
- specific things that cause high takeoff?
- -Platelet clumps
- if you have nRBCs, what do they do to the analysis, and how do you correct it?
- will cause a high-take-off. do a manual diff and correct for the nRBCs.
- if you have platelet clumps, how do you correct?
- do a manual platelet count.
- when the WBC count is over 90000 cells/uL, what happens to the analysis?
How do you correct it?
- invalidate the WBC count;
to correct, dilute the sample and rerun it.
- if the WBC count is greater than 2% of the RBCs, what does this mean?
- You must correct the RBC: subtract two percent of the RBC from the old RBC count for a new one.
- What are the effects of a high WBC count?
- -falsely elevated HgB (cloudy)
-incorrect RBC and MCV
-Hct must be spun, not calculated.
- what stain is used in normal retic counts?
- new methylene blue
- what occurs in an automated retic counter? (process)
- 1. cells are stained/incubated.
2. in weak H2SO4, cells lyse, Hgb escapes, and a ghost with precipitated RNA remains.
- What are the 2 major types of automated retic counters?
- R-3000 by Sysmex;
Advia Retic counter
- What type of dye does the sysmex use?
- Auromine-O flourescent dye.
- what type of dye does the Advia retic counter use?
- Oxazine 750
- what type of technology does the Advia retic counter use?
- Light scattering - at three angles.
- What does IRF refer to?
- the advia's zones for high and medium staining retics; was used as an indicator of whether a bone marrow graft was successful.
- what's the difference btwn the Sysmex and Advia retic counters?
- Sysmex - based on flourescent staining.
Advia - based on light scatter.
- Which analyzers use electrical impedence for counting?
- Which parameter is measured identically in all of the analyzers?
- Which analyzers use cytochemical stain?
- -Sysmex - for eos/basos
-Advia - for everything.
- Which analyzers use NO cytochemical stain?
- when would you see a dual-population of cells on the RBC histogram?
- in iron def. anemia
- a platelet histogram shifting to the right indicates:
- platelet clumping and giant platelets.
- a RBC histogram that has a high takeoff indicates:
Where would nRBCs be?
- schistocytes, platelets.
nRBCs are to the right of normal because they are BIGGER.
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