Glossary of Procedure
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- Order of microscope powers
- A. Low power
C. Oil immersion
- What are the two things you do at low power in a diff?
- 1. Determine nonrandom cell distribution - a. fringe analysis
c. platelet clumping
d. look for artifact
2. Select the best area for a diff
- What occurs at the High-dry power in a diff?
- WBC estimate.
- How do you do a WBC estimate?
- 1. Area: RBC overlapping slightly
2. Average #WBCs in 5 fields.
3. Multiply x 2000 = WBC estimate
4. See if estimate agrees with real count.
- What do you do after a WBC estimate?
- switch to oil immersion for the 100-cell diff. but FIRST DO A STAIN AND SMEAR EVAL
- what do you look for when evaluating
- Stain: excessively blue, excessively pink, or precipitated stain.
smear: Water contamination, echinocytes everywhere, thickness/length of the smear.
- What cells are not included in the 100 WBC count/id?
Giant platelet forms
- what do you do if you find a nRBC?
- correct the WBC:
100 x WBC
--------- = Corrected WBC
100 + nRBC
- what do you do when done with the 100 ID/count?
- platelet estimate.
count and avg # platelets in 6 fields, multiply by 15
- when should you repeat a diff?
- when abnormal cells are seen
nRBC's are seen
WBC < 2000/ul
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