Glossary of Mycology - Yeasts
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- Where are yeasts normal flora?
- -Oral cavity
- So how do we often see yeasts in lab?
- As Contaminants
As opportunistic infections
- What specimens can yeast contaminate?
- -Lower Resp tract
-Upper resp tract
- Yeast isolated from what specimens would indicate a pathogenic infection?
- -Blood, CSF, body fluids
-Tissues, abscess, urine, LRT
- What is included in the lab workup of a yeast?
- -Colonial morphology
-API 20C/other automated
- What types of agar are used to grow yeast?
- What is chromagar for?
- Differentiating C. albicans
- What chromagar colors are:
- Tropic = blue
Albicans = aqua
Glabrata = pink
Parapsilosis = white
- What are 4 methods for complete identification of yeasts?
- -Assimilation sugars
- What 3 disease categories are associated w C. albicans?
- 1. Mucocutaneous
- What are 4 mucocutaneous infections of C. albicans?
- 1. Thrush
- What causes intestinal mucocutaneous C. albicans infection?
- Overuse of antibiotics
- What temp is Corn meal agar incubated at?
- MUST be 25'C; 30 can inhibit.
- 4 types of cutaneous C. albicans infections are:
- 1. Intertriginous
2. Diaper rash
4. Candida granuloma
- What are 4 types of systemic C. albicans infections?
- 1. Urinary tract
- What is the more commonly isolated Candida sp from UTI?
- C. albicans; then
- What are some causes of disseminated candidiasis?
- -Numerous intravasc. devices
-More immunocompromised pts
-Technology-assisted life support
- What is a common oral thrush infection in AIDS patients?
- What do ALL candida species produce on corn meal agar?
- -Yeast cells
- What does C. albicans produce to differentiate it?
- -Terminal chlamydospores
- What are some risk factors for disseminated candidiasis?
-Severe systemic diseases
- What 3 common Antifungals are used for Candidiasis?
- -Amphotericin B deoxycholate
- What should be kept in mind regarding antifungals?
- C. albicans is developing resistance to the azoles
- What cornmeal characteristics are unique to C. glabrata?
- -Yeast cells
- How does C. glabrata look on the API 20C?
ONLY GLU/TRE pos
- What infections does C. glabrata typically cause?
-Fungemia - esp in patients with solid tumors
- What yeast is associatd with CNS disorders?
- Cryptococcus neoformans
- What other type of disease does it cause?
- pulmonary cryptococcosis
- What 4 methods are used for identifying c. neoformans?
- -India ink prep
- What do the id methods show for c. neoformans?
- India ink = capsule
Latex aggl = agglutination
Urea = pos
Birdseed = brown
- What is the birdseed agar used to show again?
- Caffeic acid in c. neoformans - it turns colonies brown on agar.
- What is a common contaminant in research labs?
- What is the identifying characteristic about Rhodotorula?
- Red colonies, urea pos
- When should amphotericin B be used as an antifungal?
- only as a last resort; it's very hard on the kidneys.
- how is c. neoformans transmitted?
- PIGEON DUNG
- What type of yeast is Blastoschizomyces?
- Normal skin flora
- What infections does Blastoschizomyces cause?
- What type of morphology does blastoschizomyces have on agar?
-Short arial hyphae
- What characteristics are used to differentiate Blastoschizomyces on agar?
- What do we differentiate Blastoschizomyces from?
- What are id characterisitcs of trichosporon?
- -Psudohyphe and Hyphae
- What are id characteristics of geotrichum?
-LOTS of arthrospores
- what is trichosporon known to cause?
- white piedra
- what is the beer/bread yeast?
- saccharomyces cerevisiae
- What a unique characteristic of S. cereviseae?
- -Ascospores - sexual spores that stain acid fast.
- What mold is a common cause of pneumonia in AIDS pts?
- -Pneumocystis jiroveci
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