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Glossary of Microbiology Bacteria identification (Strep, Staph, Neisseria, Enterococci)

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General features of most Staphylococcus species
Gram + cocci
Catalase +
ability to ferment glucose anaerobically (Facultative)
Bacitracin susceptibility -
furozolidone susceptibility +
Gram +
Catalase +
Coagulase +
Latex agglutination +
mannitol fermentation +
white colonies on SBA
yellow colonies on Mannitol Salt agar
Staphylococcus aureus
Catalase +
Gram +
Coagulase -
Novobiocin resistance +
mannitol fermentation +/-
bacitracin susceptibility -
furozolidone susceptibility +
Staphylococcus saprophyticus
Catalase +
Coagulase -
Novobiocin resistance -
mannitol fermentation -
white colonies on SBA
yellowish colonies on Mannitol Salt agar
Staphylococcus epidemidis (possibly)
Gram +
Coagulase -
anaerobic glucose fermentation -
Bacitracin susceptibility +
furozolidone susceptibility -
Micrococci
General features of most streptococci
Gram + cocci, oval/round usually in chains
Catalase -
most facultative anaerobes
non-motile
non-spore forming
grouped by appearance on SBA
(alpha, beta, gamma hemolysis)
List major alpha hemolytic strep & enterococci species
Streptococcus pneumoniae
Streptococcus bovis
Enterococcus
Viridans Streptococcus
List major beta hemolytic strep & enterococci species
S. pyogenes - GROUP A
S, agalactiae - GROUP B
S. bovis - GROUP D
Enterococcus (rare)
List major gamma hemolytic strep & enterococci species
Viridans Streptococcus
S. bovis - GROUP D
Enterococcus
B-hemolytic on SBA
Catalase -
Latex agglutination +
Bacitracin sensitivity +
PYR +
Strep pyogenes (GROUP A)
B-hemolytic SBA
Catalase -
Latex agglutination +
CAMP +
Hippurate hydrolysis +
Bacitracin sensitivity -
Streptococcus agalactiae (GROUP B)
Catalase -
Bile esculin +
6.5% NaCl -
Streptococcus bovis (GROUP D)
Lancet-shaped gram + cocci
a-hemolysis on SBA
Catalase -
Capsule (Quellung) +
Bile solubility +
Optochin sensitivity +
Streptococcus pneumoniae
alpha or gamma hemolysis on SBA
Catalase -
Bile esculin +
Growth on 6.5% NaCl +
PYR +
E. fecalis
E. faecium

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