Glossary of Comparison of Immunoassays
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- What are the 5 advantages of latex agglutination?
- 1. versatile
3. no equipment needed
4. Good screening test
5. Long-lived reagents
- What are the 2 drawbacks of latex agglutination?
- 1. Variability btwn manufacturs
2. Not quantitative.
- What are the 3 advantages of hemagg. inhibition?
- 1. Reference methodology
2. Good for IgM assays
3. Easy to read
- what are the 2 drawbacks to hemagglutination inhibition?
- 1. Time consuming
2. Varied sensitivity
- what are the 2 advantages of EMIT?
- 1. Simple to perform
2. No separation step needed
- what are 2 drawbacks of emit?
- 1. Limited to low MW antigens
2. Not as sensitive as other EIAs
- what are the qualities of ELISA?
-variety of detection systems
-good for automation
-adaptable for screening
-qualitative OR quantitative
- what are the drawbacks of ELISA?
- only cost-effective for batching
-variability of SP carriers
-some agents are carcinogenic or expensive
- what are the advantages to direct or indirect IFA?
-variety of methods
-sameday results for DFA
- what are the drawbacks to IFA?
- -background/nonspcfc fluorscn
-interpretation needs expert
- what is the advantage of RIA?
- WHAT ARE THE DRAWBACKS TO Ria?
- -Disposal of reagents
- what are the advantages of SPRIA and IRMA?
- -More stable/sensitive/rapid than plain RIA
-measures total amt of antibody
-uses less reagent than RIA
- what are the drawbacks of SPRIA and IRMA?
- same as RIA - disposal/cost
- what are the advantages of complement fixation?
- 1. Reference method
2. Relatively sensitive
3. Adaptable to many tests
- what are the drawbacks to complement fixation?
- -time consuming
-requires many controls
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