Glossary of Coag instruments
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- What is coagulation instrumentation based on?
- detection of an endpoint in the coag cascade when a clot forms.
- When are the 2 most common times you would use coag?
- 1. To monitor anticoag therapy
2. before a surgical procedure
- what are 2 common coag tests?
- PT: prothrombin time
APTT: actvtd ptl thromboplastin time
also Factor assays
- what is PT?
- the time it takes for a clot to form.
- What are 3 ways to measure coagulation?
- 1. Manual
- What is the manual method that we'll use next year called?
- What's the principle of the fibrinometer?
- 1. Automatic pipetter shoots sample into cup, time starts.
2. Heater starts and 1 probe agitates. 1 probe stationary.
3. When clot forms, agitation stops; current flows; time stops. Endpoint detected!
- What's an advantage/disadvantage of the mechanical method?
- -More precise btwn techs
-More cumbersome/frustrating method.
- What are some different photometric coag methods?
- 1. Light scatter optical
4. Absorbance /or fluorescence.
- What's a chromogenic assay based on?
- the fact that a chromophore when bound to pna doesn't shine, but when a coag factor cleaves it, it does shine. allows for factor assays.
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