Glossary of Centrifuge
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- What 3 things are centrifuges used for?
- -simulating gravity
-seperating two things with diff densities and one's a liquid
- What is the diff btn centrifugal and centripetal force?
- Centrif pulls out
Centrip is pulling in
- What 3 factors affect force?
- What's the equation for force?
- F = mrw'2
- What is RCF?
- relative centrifugal force
- what is RCF for?
- allows comparison of centrifuge force between different machines
- What's the equation for RCF?
- RCF = (1.12x10^-5)rN^2
- What factors affect settling in a centrifuge, and what law tells us that?
- Stoke's law
V = kr^2g(dp-dL)n
- Ok, in plain english please...
What 6 factors affect settling?
- -Velocity of particles
-Liquid density and viscosity
- how does particle shape alter
- the smoother, the faster.
the more dense, the faster.
the bigger, the faster.
- why do RBCs settle below WBCs in the Hct?
- RBCs are more streamlined and dense than WBCs and these factors simply carry more weight than size.
- what are the components of the electric motor in a centrifuge?
- Armature windings, field coils, carbon brushes, etc.
- What's better, a mechanical or electrical brake?
- Electrical - prevents bumping, quicker, more gradual.
- what are the 2 types of centrifuge heads?
- -Fixed angle (conical)
- What type of centrif is the hct?
- Fixed angle (conical)
- What are advantages of the fixed angle?
- -Gains higher speeds
-Particles travel less distance
-Better seperaton of supernat in shorter period of time.
- What are the disadvantages of fixed angle?
- Particles pack at an angle so you can't measure their surface.
- What are the advantages of a Horizontal (swinging) centrif?
- Allows exact measurement of sediment.
Partcls move entire tubelength, swinging tubes create air resistance.
- what are 4 clinical applications of centrifuges?
- 1. Seperation of solids/liquids
3. Special purposes
4. Centrifugal analyzer
- what are special purposes?
- -cell washing
-blood processing (during surgery)
- What are Centrifugal analyzers defined as?
- analytical instruments in clinical chemistry that use centrifugal force to transfer liquids and monitor several simultaneous chemical rxtns.
- what is the advantage of centrifugal fast spectrophotometry?
- it allows discrete analysis of each sample, seperated by an actual physical barrier at all times.
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