Glossary of CLLS124: Staph
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- What is the catalase reaction for Staphylococcus?
- Which tests which differentiate between S. aureus and the Coagulase negative Staphylococci
Thermostable nuclease test
- Identify this bacterium:
Staphylatex test positive
- Staphylococcus aureus
- What test will differentiate between S. saprophyticus and other Staphylococci?
- Novobiocin resistance
S. saprophyticus is resistant to novobiocin; other Staphylococci are susceptible.
- What is the DNAse reaction of Staphylococcus epidermidis?
Of the Staphylococci, only S. aureus is positive
- What type of coagulase is detected by the slide coagulase test?
- Bound coagulase.
The tube test detects both free and bound coagulase.
- Describe the principle of the Staphylatex test.
- Latex beads are coated with fibrinogen and anti-Protein A.
S. aureus has coagulase which converts the fibrinogen to fibrin, forming a clot. It also has the Protein A antigen -- when the antibody reacts with the antibody you get agglutination. Overall, a positive reaction is clumping.
- How does Micrococcus compare to Staphylococcus?
- Both are gpc, catalase positive.
Micrococcus has a brighter yellow pigment.
- How does Stomatococcus compare to Staphylococcus?
- Both are gpc.
Staphylococcus is catalase positive; Stomatococcus is catalase variable.
Stomatococcus colonies are sticky as compared to Staphylococcus.
- Where are these bacteria normal flora?
a. Staphylococcus aureus
b. Coagulase negative Staphylococcus
- a and b. skin and mucus membranes (mouth and vagina)
- What type of infection is associated with Staphylococcus epidermidis?
- SBE when a prosthetic heart valve is involved.
- Name diseases associated with S. aureus.
- Skin diseases (e.g. acne, folliculitis, impetigo, boils, scalded skin syndrome)
toxic shock syndrome
staph food poisoning
- What infection does S. saprophyticus cause?
- UTI in sexually active 18-25 year old females
- What is the first choice for antibiotic treatment of S. aureus?
If methicillin resistant, use vancomycin.
For VISA, GISA, VRSA strains, use Synercid or Zyvox.
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