Glossary of 5. BUN and Creat methodologies
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- List the 2 main types of BUN methodologies:
- 1. Indirect methods
2. Direct methods
- List the 3 indirect BUN methods?
- 1. Coupled enzymatic
- What is the direct BUN method?
- Diacetyl monoxime
- What is the basic principle of the indirect BUN methods?
- -Urea is converted to CO2 NH4 ion;
-The ammonium ion is then measured.
- What enzyme is used for "Coupled Enzymatic BUN method"?
What is ultimately measured?
- -Urease enzyme
-Final measurement: NAD
- What wavelength is NAD measured at?
- 340 nm
- What is ultimately measured in the Berthelot method?
- Indophenol Blue product
- Why is the Berthelot method less dependible?
- Ammonia in the air can cause contamination and nonspecific results.
- What is the principle of the Conductimetric method for BUN?
- -Ammonium carbonate is formed after urease treatment
- What is measured in the Diacetyl monoxime method for BUN?
- a yellow diazine product
- List 3 different methods for testing Creatinine levels:
- 1. Jaffe'
2. Jaffe'-Kinetic modification
- On what reaction are chemical methods for Creatinine determination based?
- Creatinine + picric acid -> orange-red chromagen
- What wavelength is the Jaffe method read at?
- between 490 and 500 nm
- What are interfering substances with the Jaffe method?
- Bilirubin, other nonspecific reactants (glucose/ascorbic acid, etc)
- What reagent can make the Jaffe reaction more specific?
- Lloyd's reagent
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